TCP1 alpha/CCTA 抗体 (C-Term) (HRP)
Quick Overview for TCP1 alpha/CCTA 抗体 (C-Term) (HRP) (ABIN2485922)
抗原
See all TCP1 alpha/CCTA (TCP1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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特异性
- Detects ~60 & ~92 kDa. Cross reactivity with human HSP60 in WB. Reacts weakly with S. cerevisiae, consistent with the epitope being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recog TCP1 of P. sativum, and the sequence of A. thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.
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交叉反应
- C. elegans, Cow, 犬, Drosophila melanogaster, 豚鼠, Hamster, 人, 猴, 小鼠, Pig, 兔, 大鼠, Saccharomyces cerevisiae
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纯化方法
- Protein G Purified
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免疫原
- Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).
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亚型
- IgG2a
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anti-T-Complex 1 (TCP1) (AA 1-556) antibody
TCP1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2B2-D6 unconjugated
anti-T-Complex 1 (TCP1) (C-Term) antibodyVerified TCP1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-T-Complex 1 (TCP1) (AA 82-98) antibodyTCP1 适用: 人, 小鼠, 大鼠 WB, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-T-Complex 1 (TCP1) (AA 406-506) antibodyTCP1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-T-Complex 1 (TCP1) (C-Term) antibody (Atto 594)TCP1 适用: 小鼠 WB, IF, FACS, IP, ICC 宿主: 大鼠 Monoclonal 91a Atto 594
anti-T-Complex 1 (TCP1) (C-Term) antibody (Atto 390)TCP1 适用: 小鼠 WB, IF, FACS, IP, ICC 宿主: 大鼠 Monoclonal 91a Atto 390
anti-T-Complex 1 (TCP1) (C-Term) antibodyTCP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-T-Complex 1 (TCP1) (AA 307-556) antibodyTCP1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-T-Complex 1 (TCP1) (C-Term) antibodyTCP1 适用: 小鼠 WB, IF, FACS, IP, ICC 宿主: 大鼠 Monoclonal 91a unconjugated
anti-T-Complex 1 (TCP1) (AA 600-680) antibodyTCP1 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-063R unconjugated Recombinant Antibody
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应用备注
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- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
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说明
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1 μg/ml of ABIN2485922 was sufficient for detection of TCP1 alpha in 20 μg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))
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别名
- TCP1 alpha
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背景
- T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40 % identity to TF55, but only minimal similarity to HSP60 and GroEL.
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基因ID
- 21454
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NCBI登录号
- NP_038714
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UniProt
- P11983
抗原
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