CAMKII gamma 抗体 (HRP)
Quick Overview for CAMKII gamma 抗体 (HRP) (ABIN2484921)
抗原
See all CAMKII gamma (CAMK2G) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Detects phosphorylated CaMKII from rat tissues. This antibody is specific for α and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β).
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Protein G Purified
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免疫原
- Synthetic peptide
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亚型
- IgG1
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anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody
CAMK2G 适用: 大鼠 WB, IHC, ELISA, IP, IF, ICC, RIA 宿主: 小鼠 Monoclonal 6G9 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) (AA 322-481) antibodyCAMK2G 适用: 人, 大鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 8G10C1 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) (AA 309-338), (C-Term) antibodyCAMK2G 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB1246 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibodyCAMK2G 适用: 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 小鼠 Monoclonal 22B1 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (HRP)CAMK2G 适用: 大鼠 WB, IHC, ELISA, IP, IF, ICC, RIA 宿主: 小鼠 Monoclonal 6G9 HRP
anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (FITC)CAMK2G 适用: 大鼠 WB, IHC, ELISA, IP, IF, ICC, RIA 宿主: 小鼠 Monoclonal 6G9 FITC
anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (Biotin)CAMK2G 适用: 大鼠 WB, IHC, ELISA, IP, IF, ICC, RIA 宿主: 小鼠 Monoclonal 6G9 Biotin
anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (PE)CAMK2G 适用: 大鼠 WB, IHC, ELISA, IP, IF, ICC, RIA 宿主: 小鼠 Monoclonal 6G9 PE
anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (PerCP)CAMK2G 适用: 大鼠 WB, IHC, ELISA, IP, IF, ICC, RIA 宿主: 小鼠 Monoclonal 6G9 PerCP
anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (APC)CAMK2G 适用: 大鼠 WB, IHC, ELISA, IP, IF, ICC, RIA 宿主: 小鼠 Monoclonal 6G9 APC
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应用备注
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- WB (1:1000)
- IHC (1:100)
- ICC/IF (1:1000)
- optimal dilutions for assays should be determined by the user.
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说明
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1 μg/ml was sufficient for detection of 0.2 μg CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- CAMKII gamma (CAMK2G) (Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G))
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别名
- CaMKII
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背景
- CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
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基因ID
- 25400
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NCBI登录号
- NP_037052
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UniProt
- P11275
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途径
- WNT signaling, Interferon-gamma Pathway, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of long-term Neuronal Synaptic Plasticity
抗原
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