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SOD2 抗体 (FITC)

This anti-SOD2 antibody (ABIN2484765) is a Rabbit Polyclonal antibody detecting SOD2 in WB, IHC, ELISA, IP, IF, ICC. Suitable for Rat.
产品编号 ABIN2484765
发货至: 中国

Quick Overview for SOD2 抗体 (FITC) (ABIN2484765)

抗原

See all SOD2 抗体
SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

适用

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大鼠

宿主

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克隆类型

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多克隆

标记

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This SOD2 antibody is conjugated to FITC

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • 特异性

    Detects ~25 kDa.

    交叉反应

    小鸡, Cow, 犬, Drosophila melanogaster, 豚鼠, Hamster, 人, Invertebrate, 猴, 小鼠, Pig, 兔, 大鼠, Sea Squirt, 绵羊, Squirrel, 非洲爪蟾

    纯化方法

    Protein A Purified

    免疫原

    Recombinant Rat Mn SOD Protein
  • 应用备注

    • WB (1:5000)
    • IHC (1:100)
    • ICC/IF (1:120)
    • optimal dilutions for assays should be determined by the user.

    说明

    0.5 μg/ml of ABIN2484765 was sufficient for detection of Mn SOD in 20 μg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Conjugated antibodies should be stored at 4°C
  • 抗原

    SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

    别名

    SOD2

    背景

    Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).

    基因ID

    24787

    NCBI登录号

    NP_058747

    UniProt

    P07895

    途径

    Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling
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