HMOX1 抗体 (HRP)
Quick Overview for HMOX1 抗体 (HRP) (ABIN2484289)
抗原
See all HMOX1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Detects ~32 kDa.
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交叉反应
- 犬, 人, 小鼠, 大鼠
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纯化方法
- Protein A Purified
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免疫原
- Human heme-oxygenase (HO-1) synthetic multiple antigenic peptide
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anti-Heme Oxygenase (Decycling) 1 (HMOX1) antibody
KO Validated HMOX1 适用: 人 WB, ELISA, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heme Oxygenase (Decycling) 1 (HMOX1) (AA 1-288) antibodyHMOX1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Heme Oxygenase (Decycling) 1 (HMOX1) antibodyHMOX1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Heme Oxygenase (Decycling) 1 (HMOX1) (AA 1-30) antibodyHMOX1 适用: 人 WB, IHC, IP, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 1F12-A6 unconjugated
anti-Heme Oxygenase (Decycling) 1 (HMOX1) (AA 1-30) antibody (HRP)HMOX1 适用: 人 WB, IHC, IP, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 1F12-A6 HRP
anti-Heme Oxygenase (Decycling) 1 (HMOX1) (N-Term) antibodyHMOX1 适用: 人, 大鼠, 小鼠, Pig, 犬, Cow, 豚鼠, 兔, 山羊 WB 宿主: 兔 Polyclonal unconjugated
anti-Heme Oxygenase (Decycling) 1 (HMOX1) (AA 99-237) antibodyHMOX1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heme Oxygenase (Decycling) 1 (HMOX1) (AA 61-172) antibodyHMOX1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal A4 unconjugated
anti-Heme Oxygenase (Decycling) 1 (HMOX1) (AA 61-172) antibodyHMOX1 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heme Oxygenase (Decycling) 1 (HMOX1) (AA 1-30) antibody (FITC)HMOX1 适用: 人 WB, IHC, IP, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 1F12-A6 FITC
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应用备注
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- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
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说明
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1 μg/ml of ABIN2484289 was sufficient for detection of HO-1 in 10 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))
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别名
- HO-1
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背景
- Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator, biliverdin and bilirubin are potent antioxidants, and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3, however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).
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基因ID
- 3162
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NCBI登录号
- NP_002124
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UniProt
- P09601
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途径
- Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome
抗原
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