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AMIGO1 抗体 (AA 554-574) (Atto 390)

This Atto 390-conjugated 小鼠 单克隆 anti-AMIGO1 antibody (Clone S86-36) specifically detects AMIGO1 in WB, IHC, ICC 和 IF. The antibody is reactive with 人 samples.
产品编号 ABIN2483801
发货至: 中国
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Quick Overview for AMIGO1 抗体 (AA 554-574) (Atto 390) (ABIN2483801)

抗原

See all AMIGO1 抗体
AMIGO1 (Adhesion Molecule with Ig-Like Domain 1 (AMIGO1))

适用

  • 53
  • 25
  • 8
  • 3
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  • 3

宿主

  • 40
  • 31
小鼠

克隆类型

  • 40
  • 31
单克隆

标记

  • 24
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  • 3
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  • 3
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  • 1
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This AMIGO1 antibody is conjugated to Atto 390

应用范围

  • 52
  • 36
  • 32
  • 22
  • 13
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  • 4
  • 3
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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

克隆位点

S86-36
  • 抗原表位

    • 23
    • 22
    • 18
    • 15
    • 8
    • 6
    • 5
    • 2
    • 1
    AA 554-574

    特异性

    Detects ~60-80 kDa depending on maturity/glycosylation.

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Protein G Purified

    免疫原

    Fusion protein amino acids 554-574 (cytoplasmic C-terminus) of human AMIGO-1

    亚型

    IgG1
  • 应用备注

    • WB (1:1000)
    • optimal dilutions for assays should be determined by the user.

    说明

    1 μg/ml of ABIN2483801 was sufficient for detection of AMIGO-1 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Conjugated antibodies should be stored at 4°C
  • 抗原

    AMIGO1 (Adhesion Molecule with Ig-Like Domain 1 (AMIGO1))

    别名

    AMIGO1

    背景

    The amphoterin-induced gene and ORF (AMIGO) family of proteins consists of AMIGO1, AMIGO2 and AMIGO3. All three members are single pass type I membrane proteins that contain several leucine-rich repeats, one IgG domain and a transmembrane domain. The AMIGO proteins are specifically expressed on fiber tracts of neuronal tissues and participate in their formation. They can form complexes with each other, but can also self-bind. AMIGO1, also designated Alivin2, promotes growth and fasciculation of neurites and plays a role in myelination and fasciculation of developing neural axons. In cerebellar neurons, AMIGO2 (Alivin1) is crucial for depolarization-dependent survival. Similar to AMIGO1 and AMIGO2, AMIGO3 (Alivin3) plays a role in hemophilic and/or heterophilic cell-cell interaction and signal transduction.

    基因ID

    57463

    NCBI登录号

    NP_065754

    UniProt

    Q86WK6
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