DNAJB1 抗体 (FITC)
Quick Overview for DNAJB1 抗体 (FITC) (ABIN2481541)
抗原
See all DNAJB1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Detects ~40 kDa. Yeast specific product. Does not cross react with Human, Mouse or Rat.
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交叉反应
- Saccharomyces cerevisiae
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纯化方法
- Protein G Purified
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免疫原
- Full length protein yeast HSP40 (YDJ1)
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亚型
- IgG1 kappa
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anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (C-Term) antibody
DNAJB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (AA 1-340) antibodyDNAJB1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (AA 2-340) antibodyDNAJB1 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (AA 1-340) antibodyDNAJB1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibodyDNAJB1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibodyDNAJB1 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 小鼠 Monoclonal 3B9-E6 unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibodyDNAJB1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB1770 unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (AA 74-123) antibodyDNAJB1 适用: 人, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibodyDNAJB1 适用: Saccharomyces cerevisiae WB, ELISA, IP 宿主: 小鼠 Monoclonal 2A7-H6 unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibody (HRP)DNAJB1 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 小鼠 Monoclonal 3B9-E6 HRP
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应用备注
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- WB (1:2000)
- optimal dilutions for assays should be determined by the user.
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说明
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0.5 μg/ml of ABIN2481541 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
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别名
- HSP40
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背景
- Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of HSP40, type 1 DNAJ proteins including HDJ2 and yeast YdjI, type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not. Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (5, 6). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).
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基因ID
- 855661
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NCBI登录号
- NP_014335
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UniProt
- P25491
抗原
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