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DNAJB1 抗体 (FITC)

This 小鼠 单克隆 antibody specifically detects DNAJB1 in WB, ELISA 和 IP. It exhibits reactivity toward Saccharomyces cerevisiae.
产品编号 ABIN2481541
发货至: 中国
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Quick Overview for DNAJB1 抗体 (FITC) (ABIN2481541)

抗原

See all DNAJB1 抗体
DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

适用

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Saccharomyces cerevisiae

宿主

  • 59
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小鼠

克隆类型

  • 59
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单克隆

标记

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This DNAJB1 antibody is conjugated to FITC

应用范围

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Western Blotting (WB), ELISA, Immunoprecipitation (IP)

克隆位点

1G10-H8
  • 特异性

    Detects ~40 kDa. Yeast specific product. Does not cross react with Human, Mouse or Rat.

    交叉反应

    Saccharomyces cerevisiae

    纯化方法

    Protein G Purified

    免疫原

    Full length protein yeast HSP40 (YDJ1)

    亚型

    IgG1 kappa
  • 应用备注

    • WB (1:2000)
    • optimal dilutions for assays should be determined by the user.

    说明

    0.5 μg/ml of ABIN2481541 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Conjugated antibodies should be stored at 4°C
  • 抗原

    DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

    别名

    HSP40

    背景

    Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of HSP40, type 1 DNAJ proteins including HDJ2 and yeast YdjI, type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not. Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (5, 6). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).

    基因ID

    855661

    NCBI登录号

    NP_014335

    UniProt

    P25491
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