Complement C4d 抗体
(2 references)Quick Overview for Complement C4d 抗体 (ABIN2477810)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Mouse anti Human C4d antibody, clone 10-11 recognizes the secreted protein complement component 4d (C4d). The presence of C4d in renal peritubular capillaries is a key indicator for acute antibody-mediated rejection [AMR] (Collins et al. 1999.).
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无交叉反应
- 猫, 牛, 犬, 豚鼠, 小鼠, 兔, 大鼠, 绵羊
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产品特性
- Mouse anti Human C4d antibody, clone 10-11 recognizes complement component 4d. The presence of C4d in renal peritubular capillaries is a key indicator for acute antibody-mediated rejection. C4d is a footprint of complement activation, making it a perfect maker of allograft rejection. Mouse anti Human C4d antibody, clone 10-11 is used to detect the biomarker C4d which has been described as a “footprint” of antibody mediated tissue rejection (Sacks and Chowdhury 2002). The internal thioester of C4b becomes exposed during cleavage to C4d and forms a covalent bond with the cell surface. The longer half-life of covalently bound C4d makes it a footprint of complement activation long after weakly bound antibodies have been cleared by the blood stream (Sacks and Chowdhury 2002).
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纯化方法
- Purified IgG prepared by affinity chromatography on Protein A
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亚型
- IgG1
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anti-Complement Component C4d (C4d) (Internal Region) antibody
C4d 适用: 人 IHC (p) 宿主: 兔 Monoclonal A24-T unconjugated
anti-Complement Component C4d (C4d) antibodyC4d 适用: 人 IF, ELISA, Coat, IHC (f) 宿主: 小鼠 Monoclonal C4D204 unconjugated
anti-Complement Component C4d (C4d) (AA 958-1335) antibodyC4d 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component C4d (C4d) (AA 958-1335) antibodyC4d 适用: 人 IHC, WB, IP, ICC 宿主: 小鼠 Monoclonal D2 unconjugated
anti-Complement Component C4d (C4d) (AA 1241-1256) antibodyC4d 适用: 人 IF, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Complement Component C4d (C4d) antibodyC4d 适用: 人 IF, ELISA, IHC (p) 宿主: 小鼠 Monoclonal C4D204 unconjugated
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应用备注
- ELISA: 1/5,000-1/20,000 IF: 1/250-1/600 IHC (frozen): 1/100-1/750 It has been reported that this antibody works very well on acetone-fixed, frozen renal biopsies. Strong staining is observed in the glomeruli and in some cases the peritubular capillaries. Clone 10-11 has given variable results on formalin-fixed, paraffin-embedded sections. It has been observed that pre-treatment with 88% formic acid for 20 minutes at room temperature is beneficial
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- Borate buffered saline, 0.09% Sodium Azide (NaN3)
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注意事项
- Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted.
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: "Acute humoral rejection in kidney transplantation: II. Morphology, immunopathology, and pathologic classification." in: Journal of the American Society of Nephrology : JASN, Vol. 13, Issue 3, pp. 779-87, (2002) (PubMed).
: "Temporal accrual of complement proteins in amyloid plaques in Down's syndrome with Alzheimer's disease." in: The American journal of pathology, Vol. 156, Issue 2, pp. 489-99, (2000) (PubMed).
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: "Acute humoral rejection in kidney transplantation: II. Morphology, immunopathology, and pathologic classification." in: Journal of the American Society of Nephrology : JASN, Vol. 13, Issue 3, pp. 779-87, (2002) (PubMed).
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- Complement C4d (C4d) (Complement Component C4d (C4d))
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别名
- C4d
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背景
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Synonyms: COMPLEMENT COMPONENT 4d
C4d was accepted in 2003 into the Banff classification for identification of acute AMR (Racusen et al. 2003). Mouse anti Human C4d antibody, clone 10-11 is specific for C4d, a marker that can be used in the detection of acute AMR for kidney, heart, pancreas and lung allografts. C4d is regarded as a key marker of antibody-mediated cell injury and humoral rejection (Sacks and Chowdhury 2002). Complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b’s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4b’s short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation (Collins et al. 1999). C4 has also been linked to susceptibility to systemic lupus erythematosus (Yang et al. 2004) and rheumatoid arthritis (Makinde et al. 1989). -
基因ID
- 720, 721
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UniProt
- P0C0L4, P0C0L5
抗原
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