BrdU 抗体
(1 reference)
(2 validations)Quick Overview for BrdU 抗体 (ABIN2477713)
抗原
See all BrdU 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- Mouse anti BrdU antibody, clone Bu20a, recognizes BrdU and other thymidine analogs; 5′-chloro-2′-deoxyuridine (CldU), 5′-iodo-2′-deoxyuridine (IdU) and 2′-deoxy-5-ethynyluridine (EdU), but only shows minimal reactivity with thymidine itself
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纯化方法
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
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免疫原
- Bromodeoxyuridine conjugated to BSA
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亚型
- IgG1
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anti-Bromodeoxyuridine (BrdU) antibody
BrdU IHC (p), IF (cc), IF (p), IHC (fro), WB 宿主: 小鼠 Monoclonal A7 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), IF (cc), IF (p), IHC (fro), ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, FACS, IF, WB, ELISA, FM, DB 宿主: 小鼠 Monoclonal 29G6-E8 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, Neut, StM 宿主: 小鼠 Monoclonal BRD469 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), ICC, IF (cc), IF (p), IHC (fro), ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 小鼠 Monoclonal 4B6C12 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, Neut, StM 宿主: 兔 Monoclonal BRD2888R unconjugated Recombinant Antibody
anti-Bromodeoxyuridine (BrdU) antibody (PE)BrdU ICFC 宿主: 小鼠 Monoclonal Bu20a PE
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应用备注
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Flow: 1/25-1/100, ICC 1/25-1/100
For Flow Cytometry use 10 μl of the suggested working dilution to label 1x106 cells in 100 μl -
限制
- 仅限研究用
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- by
- Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #103189
- 日期
- 2018.10.05
- 抗原
- BrdU
- Lot Number
- 1710
- Method validated
- Immunohistochemistry
- Positive Control
Adult (24 months) mouse brain and intestine (duodenum)
- Negative Control
We incubated slices overnight with the blocking solution only and then processed them with the secondary antibody.
- Notes
Passed. ABIN2477713 works well in IHC-P, but with higher concentrations than the ones suggested by the manufacturer.
- Primary Antibody
- ABIN2477713
- Secondary Antibody
- goat anti-mouse AF488 conjugated antibody (Thermo Fisher Scientific, A11029, lot 1942237)
- Full Protocol
- Inject mouse intraperitoneally at the dose of 0.1 mg/g body weight with BrdU (Sigma, B5002) dissolved in sterile distilled water at final concentration of 100mg/ml.
- Perfuse mouse was perfused with paraformaldehyde 4% in 0.1M phosphate buffer (PB) pH 7.4 and post-fix samples in the same fixative for an additional 2h at RT.
- Wash, dehydrate, and embed samples in paraffin wax.
- Following several washes in PBS, cut intestines and brain with a microtome (6µm-thick sections) and mount sections on glass slides.
- After paraffin removal, process sections by microwave antigen retrieval for 10min (95-100°C) in 10mM sodium citrate buffer (pH6.0).
- Let sections cool for 20min.
- Wash sections 2x 5min with distilled water.
- Wash sections 5min with PBS.
- Incubate sections in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) for 1h at RT to block non-specific binding sites.
- Incubate sections with primary antibody mouse anti-BrdU (antibodies-online, ABIN2477713, lot 1710) diluted 1:10 in PBS-BSA-PLL ON at RT.
- Wash sections 3x 5min with 0.01M PBS.
- Incubate sections with the secondary goat anti-mouse AF488 conjugated antibody (Thermo Fisher Scientific, A11029, lot 1942237) diluted 1:200 in 0.1M PBS for 1h at RT.
- Wash sections 3x 5min with 0.01M PBS.
- Mount sections in Fluoroshield (Sigma, F6182, lot MKCB0153V).
- Acquire images with Leica DM 6000B fluorescence microscope equipped with the manufacturer’s FITC filter set and digital camera at magnifications of 20x and 40x. Parameters for image acquisition (Exposure 2.2s, Gain 2.3x, Saturation 1.5, Gamma 1.10) were maintained unchanged for all images.
- Experimental Notes
For the incubation with the primary antibody ABIN2477713 1:25, 1:20, 1:15 dilutions were also tested with also 0.01M PBS - 5% Normal Goat Serum (NGS Sigma, G9023, lot SLBV1396) and 0.1% Triton-X -100 as the blocking solution, but 1:10 and PBS containing 1% albumin from chicken egg white and 0.3% Triton-X gave the best results.
生效 #103189 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Staining of mouse brain and intestine sections using ABIN2477713. A-C: A bright staining in the cell nuclei of individual cells scattered across the brain is present, especially in the hippocampus, ventricular wall (A) and sub-cortical areas at the border between the gray and white matters (B-C). Some positive cells are clearly M-phase cells (C). Abbreviations: GM = gray matter; LV = lateral ventricle; WM = white matter. D-E: positive control staining in the intestine (duodenum). BrdU+ proliferating cells are clearly detected in the germinal layer of the intestinal epithelium, at the basis of the duodenal villi.]()
Staining of mouse brain and intestine sections using ABIN2477713. A-C: A bright staining in the cell nuclei of individual cells scattered across the brain is present, especially in the hippocampus, ventricular wall (A) and sub-cortical areas at the border between the gray and white matters (B-C). Some positive cells are clearly M-phase cells (C). Abbreviations: GM = gray matter; LV = lateral ventricle; WM = white matter. D-E: positive control staining in the intestine (duodenum). BrdU+ proliferating cells are clearly detected in the germinal layer of the intestinal epithelium, at the basis of the duodenal villi.
Full Methods - by
- Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #103975
- 日期
- 2018.07.05
- 抗原
- BrdU
- Lot Number
- 1807
- Method validated
- Immunohistochemistry
- Positive Control
Adult (24 months) mouse brain and intestine (duodenum)
- Negative Control
We incubated slices overnight with the blocking solution only and then processed them with the secondary antibody.
- Notes
Passed. ABIN2477713 works well in IHC-P on murine intestinal samples at a 1:10 dilution.
- Primary Antibody
- ABIN2477713
- Secondary Antibody
- goat anti-mouse AF488 conjugated antibody (Thermo Fisher Scientific, A11029, lot 1942237)
- Full Protocol
- Inject mouse intraperitoneally at the dose of 0.1mg/g body weight with BrdU (Sigma, B5002) dissolved in sterile distilled water at final concentration of 100mg/ml.
- Perfuse mouse with 4% paraformaldehyde in 0.1M phosphate buffer (PB) pH7.4 and post-fix samples in the same fixative for an additional 2h at RT.
- Wash, dehydrate, and embed samples in paraffin wax.
- Following several washes in PBS, cut intestines and brain with a microtome (6µm-thick sections) and mount sections on glass slides.
- After paraffin removal, process sections by microwave antigen retrieval for 10min (95-100°C) in 10mM sodium citrate buffer pH6.0.
- Let sections cool for 20min.
- Wash sections 2x 5min with distilled water.
- Wash sections 5min with PBS.
- Block sections in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) for 1h at RT to block non-specific binding sites.
- Incubate sections with primary mouse anti-BrdU antibody (antibodies-online, ABIN2477713, lot 1807) diluted 1:10 in PBS-BSA-PLL ON at RT.
- Wash sections 3x 5min with 0.01M PBS.
- Incubate sections with secondary goat anti-mouse AF488-conjugated antibody (Invitrogen, A11029, lot 1942237) for 1h at RT.
- Wash sections 3x 5min with 0.01M PBS.
- Mount sections in Fluoroshield (Sigma, F6182, lot MKCB0153V).
- Acquire images with Leica DM 6000B fluorescence microscope equipped with the manufacturer’s FITC filter set and digital camera at magnifications of 20x and 40x. Maintain parameters for image acquisition (Exposure 2.2s, Gain 2.3x, Saturation 1.5, Gamma 1.10) unchanged for all images.
- Experimental Notes
For the incubation with the primary antibody ABIN2477713 dilutions 1:25, 1:20, and 1:15 were also tested, but 1:10 gave the best results.
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline, 0.09%Sodium Azide (NaN3)
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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注意事项
- This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended.
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有效期
- 18 months
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: "Perinatal lipid nutrition alters early intestinal development and programs the response to experimental colitis in young adult rats." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 6, pp. G1376-85, (2010) (PubMed).
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: "Perinatal lipid nutrition alters early intestinal development and programs the response to experimental colitis in young adult rats." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 6, pp. G1376-85, (2010) (PubMed).
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- BrdU (Bromodeoxyuridine (BrdU))
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别名
- BrdU
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物质类
- Chemical
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背景
- Synonyms: 5-BROMODEOXYURIDINE
抗原
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Staining in the mouse intestine (duodenum) with mouse anti-BrdU antibody ABIN2477713.
