Western Blotting (WB), Immunohistochemistry (IHC), ELISA
纯化方法
SIRT4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
SIRT4 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of SIRT4.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 38 kDa band observed in human kidney and U937 lysates (calculated MW of 35.2 kDa according to NP_036372.1). In transfected HEK293 transiently expressing SIRT4 a band of approximately 38 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded skeletal muscle shows spotty staining of striated fibres in longitudinal section. Recommended concentration, 2-3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.