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SIRT4 抗体 (AA 51-150)

Name: SIRT4 抗体 (AA 51-150)
SKU: ABIN872709
Availability: OutOfStock

SIRT4 适用: 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

Mouse cerebrum lysates probed with SIRT4 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Paraformaldehyde-fixed, paraffin embedded rat stomach tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-SIRT4 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-SIRT4 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

Mouse liver lysates probed with SIRT4 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

4 images

(2 references)

产品编号 ABIN872709

发货至: 中国

Datasheet as PDF

抗原 See all SIRT4 抗体

SIRT4 (Sirtuin 4 (SIRT4))
抗原表位
12

11

8

6

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1
AA 51-150
适用
51

34

24

5

4

4

3

3

3

2

1

1

1

1

1

1
小鼠, 大鼠
宿主
50

8

2

1
兔
Compatible Secondaries
克隆类型
55

6
多克隆
标记
30

10

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1
This SIRT4 antibody is un-conjugated
应用范围
56

26

15

4

4

4

3

2

2

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

交叉反应

小鼠, 大鼠

预测反应

Human,Cow,Sheep,Pig,Horse,Rabbit,Zebrafish,Drosophila

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human SIRT4/SIR 2 like protein 4

亚型

IgG

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anti-Sirtuin 4 (SIRT4) (C-Term) antibody
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anti-Sirtuin 4 (SIRT4) (Middle Region) antibody
SIRT4 适用: 人, 小鼠, 大鼠, Cow, 犬, Pig, 马, 兔, 绵羊, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Sirtuin 4 (SIRT4) (AA 29-260) antibody
SIRT4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Sirtuin 4 (SIRT4) (AA 26-333) antibody
SIRT4 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sirtuin 4 (SIRT4) (AA 215-314) antibody
SIRT4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C8 unconjugated

anti-Sirtuin 4 (SIRT4) (AA 216-265) antibody
SIRT4 适用: 人, 犬, Pig, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Sirtuin 4 (SIRT4) (N-Term) antibody
SIRT4 适用: 人, 小鼠, 大鼠, Cow, 犬, Pig, 马, 兔, 绵羊, 豚鼠, 山羊, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Takumida, Takumida, Katagiri, Anniko: "Localization of sirtuins (SIRT1-7) in the aged mouse inner ear." in: Acta oto-laryngologica, pp. 1-12, (2015) (PubMed).

Takumida, Takumida, Anniko: "Localization of sirtuins in the mouse inner ear." in: Acta oto-laryngologica, Vol. 134, Issue 4, pp. 331-8, (2014) (PubMed).
抗原

SIRT4 (Sirtuin 4 (SIRT4))

别名

SIRT4 (SIRT4 产品)

别名

SIRT4 antibody, im:7139453 antibody, SIR2L4 antibody, 4930596O17Rik antibody, sirtuin 4 antibody, SIRT4 antibody, sirt4 antibody, Sirt4 antibody

背景

Synonyms: SIR2L4, NAD-dependent protein deacetylase sirtuin-4, NAD-dependent ADP-ribosyltransferase sirtuin-4, Regulatory protein SIR2 homolog 4, SIR2-like protein 4, SIRT4
Background: Acts both as NAD-dependent protein ADP-ribosyl transferase and NAD-dependent protein deacetylase. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest. In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor. Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis. Down-regulates insulin secretion.

基因ID

23409

UniProt

Q9Y6E7

途径

Negative Regulation of Hormone Secretion