MAPKAP Kinase 5 抗体 (truncated)
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- 抗原 See all MAPKAP Kinase 5 (MAPKAPK5) 抗体
- MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
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抗原表位
- truncated
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MAPKAP Kinase 5 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 免疫原
- Ni-NTA purified truncated recombinant PRAK-His expressed in E. Coli strain BL21 (DE3).
- 克隆位点
- 7H10B4
- 亚型
- IgG1
- Top Product
- Discover our top product MAPKAPK5 Primary Antibody
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- 应用备注
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Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Ascitic fluid containing 0.03 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- 储存条件
- -20 °C
- 储存方法
- PRAK monoclonal antibody can be stored at -20 °C, stable for one year.
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- 抗原
- MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
- 别名
- PRAK (MAPKAPK5 产品)
- 别名
- MAPKAPK5 antibody, MK5 antibody, PRAK antibody, MAPKAP-K5 antibody, MK-5 antibody, mitogen-activated protein kinase-activated protein kinase 5 antibody, MAP kinase-activated protein kinase 5 antibody, MAPKAPK5 antibody, mapkapk5 antibody, Mapkapk5 antibody
- 背景
- PRAK (p38-regulated /activated kinase), also referred to as mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK)-5, is an ubiquitously expressed serine/threonine kinase regulated by p38alpha and p38beta MAP kinases. Activated JNK, p38? or p38? are unable to induce phosphorylation of PRAK in vitro. Phosphorylation of PRAK occurs in vivo in response to p38 activation by stress-related extracellular stimuli including UV light, oxidation and proinflammatory cytokines. Two other substrates for p38, MAPKAPK-2 and MAPKAPK-3/3pK, share approximately 45 % sequence homology with PRAK including the phosphorylation motif recognized by p38, Lys-X-Thr-Pro. Activated PRAK has been shown to specifically phosphorylate HSP 27 in vitro, suggesting that the protein may play a role in stress-induced small heat shock protein phosphorylation in vivo.
- 基因ID
- 8550
- UniProt
- Q8IW41
- 途径
- MAPK Pathway
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