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NONO 抗体

The 兔 多克隆 anti-NONO antibody (ABIN2462238) specifically detects NONO in WB 和 ELISA. The antibody is reactive with 人, 小鼠, 大鼠 和 犬 samples.
产品编号 ABIN2462238
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for NONO 抗体 (ABIN2462238)

抗原

See all NONO 抗体
NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

适用

  • 86
  • 46
  • 42
  • 15
  • 11
  • 6
  • 5
  • 5
  • 5
  • 3
  • 3
  • 1
人, 小鼠, 大鼠, 犬

宿主

  • 80
  • 4
  • 2
  • 1

克隆类型

  • 61
  • 26
多克隆

标记

  • 43
  • 6
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This NONO antibody is un-conjugated

应用范围

  • 67
  • 43
  • 27
  • 22
  • 17
  • 14
  • 14
  • 13
  • 10
  • 6
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA
  • 纯化方法

    Antibody is purified by protein A chromatography method.

    免疫原

    Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NONO.
  • 应用备注

    NONO antibody can be used for detection of NONO by ELISA at 1:312500. NONO antibody can be used for detection of NONO by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

    浓度

    1 mg/mL

    缓冲液

    Antibody is lyophilized in PBS buffer with 2 % sucrose.

    注意事项

    As with any antibody avoid repeat freeze-thaw cycles.

    储存条件

    4 °C/-20 °C

    储存方法

    For short periods of storage (days) store at 4 °C. For longer periods of storage, store NONO antibody at -20 °C.
  • 抗原

    NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

    别名

    NONO

    背景

    NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V (D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.

    分子量

    52 kDa

    基因ID

    4841

    NCBI登录号

    NP_031389

    UniProt

    Q15233
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