KEAP1 抗体
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北京 101111
Quick Overview for KEAP1 抗体 (ABIN2461036)
抗原
See all KEAP1 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Antibody is purified by peptide affinity chromatography method.
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免疫原
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KEAP1.
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应用备注
- KEAP1 antibody can be used for detection of KEAP1 by ELISA at 1:312500. KEAP1 antibody can be used for detection of KEAP1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
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注意事项
- As with any antibody avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store KEAP1 antibody at -20 °C.
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- KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))
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别名
- KEAP1
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背景
- KEAP1 contains KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
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分子量
- 70 kDa, 70 kDa
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基因ID
- 9817
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NCBI登录号
- NP_987096
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UniProt
- Q14145
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途径
- Maintenance of Protein Location
抗原
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