This anti-SARS-CoV Spike antibody is a 小鼠 单克隆 antibody detecting SARS-CoV Spike in ELISA, WB, IF 和 IC. Suitable for SARS Coronavirus (SARS-CoV). This Primary Antibody has been cited in 5+ publications.
Monoclonal antibody 3A2 recognizes the spike protein consisting of 1181 amino acids, which migrates at 200 kDa position on SDS-PAGE due to its glyco-chains.
Jin, Nesbitt, Yang, Chen, Horowitz, Jones, Vandergaast, Carey, Reiter, Russell, Kyratsous, Hooper, Hamilton, Ferreira, Deng, Straus, Baras, Hillyer, Luchsinger: "Seroprevalence of anti-SARS-CoV-2 antibodies in a cohort of New York City metro blood donors using multiple SARS-CoV-2 serological assays: Implications for controlling the epidemic and "Reopening"." in: PloS one, Vol. 16, Issue 4, pp. e0250319, (2021) (PubMed).
Nesbitt, Jin, Hogan, Yang, Chen, Chan, Simon, Vargas, King, Huard, Bandy, Hillyer, Luchsinger: "Low Seroprevalence of SARS-CoV-2 in Rhode Island blood donors during may 2020 as determined using multiple serological assay formats." in: BMC infectious diseases, Vol. 21, Issue 1, pp. 871, (2021) (PubMed).
Satija, Lal: "The molecular biology of SARS coronavirus." in: Annals of the New York Academy of Sciences, Vol. 1102, pp. 26-38, (2007) (PubMed).
Yamate, Yamashita, Goto, Tsuji, Li, Warachit, Yunoki, Ikuta: "Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus." in: Microbes and infection / Institut Pasteur, Vol. 7, Issue 15, pp. 1530-40, (2005) (PubMed).
抗原
SARS-CoV Spike (SARS-CoV S)
(SARS-Coronavirus Spike Protein (SARS-CoV S))
别名
SARS-CoV Spike
物质类
Viral Protein
背景
A novel type of coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Spike glycoprotein is essential for the infection and directly binds to the virus receptor, ACE2 (Angiotensin-Converting Enzyme 2). Hybridoma 3A2 has been isolated by injecting mouse with SARS virus and as the clone which produces antibody that specifically reacts with the virus-infected cell, in the laboratory of Prof. K. Ikuta of Osaka University.