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SARS-CoV Spike 抗体

This anti-SARS-CoV Spike antibody is a 小鼠 单克隆 antibody detecting SARS-CoV Spike in ELISA, WB, IF 和 IC. Suitable for SARS Coronavirus (SARS-CoV). This Primary Antibody has been cited in 5+ publications.
产品编号 ABIN2452119
发货至: 中国

Quick Overview for SARS-CoV Spike 抗体 (ABIN2452119)

抗原

See all SARS-CoV Spike (SARS-CoV S) 抗体
SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))

适用

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SARS Coronavirus (SARS-CoV)

宿主

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  • 1
小鼠

克隆类型

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单克隆

标记

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This SARS-CoV Spike antibody is un-conjugated

应用范围

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ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

克隆位点

3A2
  • 产品特性

    Monoclonal antibody 3A2 recognizes the spike protein consisting of 1181 amino acids, which migrates at 200 kDa position on SDS-PAGE due to its glyco-chains.

    纯化方法

    Purified

    过滤

    Sterile filtered

    亚型

    IgG2b kappa
  • 应用备注

    1) Western blotting: 0. 1~0. 3 g/mL
    2) Immunofluoresece staining (IHC)
    3) ELISA Isoform: IgG2b (kappa)

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS, 50 % glycerol

    储存液

    Azide free

    储存条件

    -20 °C/-80 °C

    储存方法

    -20 C (For long term storage: -80 C)
  • Jin, Nesbitt, Yang, Chen, Horowitz, Jones, Vandergaast, Carey, Reiter, Russell, Kyratsous, Hooper, Hamilton, Ferreira, Deng, Straus, Baras, Hillyer, Luchsinger: "Seroprevalence of anti-SARS-CoV-2 antibodies in a cohort of New York City metro blood donors using multiple SARS-CoV-2 serological assays: Implications for controlling the epidemic and "Reopening"." in: PloS one, Vol. 16, Issue 4, pp. e0250319, (2021) (PubMed).

    Nesbitt, Jin, Hogan, Yang, Chen, Chan, Simon, Vargas, King, Huard, Bandy, Hillyer, Luchsinger: "Low Seroprevalence of SARS-CoV-2 in Rhode Island blood donors during may 2020 as determined using multiple serological assay formats." in: BMC infectious diseases, Vol. 21, Issue 1, pp. 871, (2021) (PubMed).

    Luchsinger, Ransegnola, Jin, Muecksch, Weisblum, Bao, George, Rodriguez, Tricoche, Schmidt, Gao, Jawahar, Pal, Schnall, Zhang, Strauss, Yazdanbakhsh, Hillyer, Bieniasz, Hatziioannou: "Serological Assays Estimate Highly Variable SARS-CoV-2 Neutralizing Antibody Activity in Recovered COVID19 Patients." in: Journal of clinical microbiology, (2020) (PubMed).

    Satija, Lal: "The molecular biology of SARS coronavirus." in: Annals of the New York Academy of Sciences, Vol. 1102, pp. 26-38, (2007) (PubMed).

    Yamate, Yamashita, Goto, Tsuji, Li, Warachit, Yunoki, Ikuta: "Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus." in: Microbes and infection / Institut Pasteur, Vol. 7, Issue 15, pp. 1530-40, (2005) (PubMed).

  • 抗原

    SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))

    别名

    SARS-CoV Spike

    物质类

    Viral Protein

    背景

    A novel type of coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Spike glycoprotein is essential for the infection and directly binds to the virus receptor, ACE2 (Angiotensin-Converting Enzyme 2). Hybridoma 3A2 has been isolated by injecting mouse with SARS virus and as the clone which produces antibody that specifically reacts with the virus-infected cell, in the laboratory of Prof. K. Ikuta of Osaka University.
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