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SARS-CoV Spike 抗体 (AA 532-562)

Name: SARS-CoV Spike 抗体 (AA 532-562)
SKU: ABIN1882128
Availability: OutOfStock

SARS-CoV S 适用: SARS Coronavirus (SARS-CoV) WB 宿主: 兔 Polyclonal RB3983-3984 unconjugated

The anti-SARS-Sm Pab (ABIN1882128 and ABIN2840581) is used in Western blot to detect recombinant Spike proteins, aa17-537 (Lane 1) and aa17-756 (Lane 2).

1 image

(8 references)

产品编号 ABIN1882128

发货至: 中国

Datasheet as PDF

抗原 See all SARS-CoV Spike (SARS-CoV S) 抗体

SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))
抗原表位
15

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 532-562
适用
43

16
SARS Coronavirus (SARS-CoV)
宿主
40

3
兔
Compatible Secondaries
克隆类型
40

3
多克隆
标记
27

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This SARS-CoV Spike antibody is un-conjugated
应用范围
34

26

3

1

1

1

1

1

1

1
Western Blotting (WB)

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Purified Rabbit Polyclonal Antibody (Pab)

免疫原

This SARS virus Sm antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 532-562 amino acids from the middle of SARS CoV Spike protein.

克隆位点

RB3983-3984

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应用备注

WB: 1:1000

限制

仅限研究用
状态

Liquid

浓度

2 mg/mL

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Lee, Poo, Han, Hong, Kim, Cho, Kim, Sung, Kim: "Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice." in: Journal of virology, Vol. 80, Issue 8, pp. 4079-87, (2006) (PubMed).

Ito, Mossel, Narayanan, Popov, Huang, Inoue, Peters, Makino: "Severe acute respiratory syndrome coronavirus 3a protein is a viral structural protein." in: Journal of virology, Vol. 79, Issue 5, pp. 3182-6, (2005) (PubMed).

Chan, Wu, Chow, Cheung, To, Leung, Chan, Lee, Ng, Au, Lo: "Coronaviral hypothetical and structural proteins were found in the intestinal surface enterocytes and pneumocytes of severe acute respiratory syndrome (SARS)." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 18, Issue 11, pp. 1432-9, (2005) (PubMed).

Pogrebnyak, Golovkin, Andrianov, Spitsin, Smirnov, Egolf, Koprowski: "Severe acute respiratory syndrome (SARS) S protein production in plants: development of recombinant vaccine." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 25, pp. 9062-7, (2005) (PubMed).

He, Dobie, Ballantine, Leeson, Li, Bastien, Cutts, Andonov, Cao, Booth, Plummer, Tyler, Baker, Li: "Analysis of multimerization of the SARS coronavirus nucleocapsid protein." in: Biochemical and biophysical research communications, Vol. 316, Issue 2, pp. 476-83, (2004) (PubMed).

Hogan, Gao, Rowe, Bell, Flieder, Paragas, Kobinger, Wivel, Crystal, Boyer, Feldmann, Voss, Wilson: "Resolution of primary severe acute respiratory syndrome-associated coronavirus infection requires Stat1." in: Journal of virology, Vol. 78, Issue 20, pp. 11416-21, (2004) (PubMed).

Song, Seo, Stadler, Yoo, Choo, Coates, Uematsu, Harada, Greer, Polo, Pileri, Eickmann, Rappuoli, Abrignani, Houghton, Han: "Synthesis and characterization of a native, oligomeric form of recombinant severe acute respiratory syndrome coronavirus spike glycoprotein." in: Journal of virology, Vol. 78, Issue 19, pp. 10328-35, (2004) (PubMed).

Snijder, Bredenbeek, Dobbe, Thiel, Ziebuhr, Poon, Guan, Rozanov, Spaan, Gorbalenya: "Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage." in: Journal of molecular biology, Vol. 331, Issue 5, pp. 991-1004, (2003) (PubMed).
抗原

SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))

Abstract

SARS-CoV S 产品

物质类

Viral Protein

背景

An outbreak of atypical pneumonia, referred to as severe acute respiratory syndrome (SARS) and first identified in Guangdong Province, China, has spread to several countries. The severity of this disease is such that the mortality rate appears to be approx. 3 to 6%. A number of laboratories worldwidehave undertaken the identification of the causative agent. The National Microbiology Laboratory in Canada obtained the Tor2 isolate from a patient in Toronto, and succeeded in growing a coronavirus-like agent in African Green Monkey Kidney (Vero E6) cells. This coronavirus has been named publicly by the World Health Organization and member laboratories as SARS virus. The SARS membrane proteins, including the major proteins S (Spike) and M (Membrane), are inserted into the endoplasmic reticulum Golgi intermediate compartment (ERGIC) while full length replicated RNA (+ strands) assemble with the N (nucleocapsid) protein. The virus then migrates through the Golgi complex and eventually exits the cell, likely by exocytosis. The site of viral attachment to the host cell resides within the S protein. Oligomeric spike (S) glycoproteins extend from SARS membranes. These integral membrane proteins assemble within the endoplasmic reticulum of infected cells and are subsequently endoproteolyzed in the Golgi, generating noncovalently associated S1 and S2 fragments. Once on the surface of infected cells and virions, peripheral S1 fragments bind carcinoembryonic antigen-related cell adhesion molecule (CEACAM) receptors, and this triggers membrane fusion reactions mediated by integral membrane S2 fragments.

Synonyms: S, Spike glycoprotein, E2, Peplomer protein, Spike protein S1, Spike protein S2

UniProt

P59594