RAD18 抗体 (C-Term)
(3 references)Quick Overview for RAD18 抗体 (C-Term) (ABIN2452086)
抗原
See all RAD18 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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交叉反应 （详细）
- Not reactive to mouse Rad18
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纯化方法
- Affinity purified with His-tagged Rad18 protein affinity column
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免疫原
- GST-fusion protein containing 113 carboxyl terminal of human Rad18
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亚型
- IgG
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anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 332-430) antibody
RAD18 适用: 人 WB, ELISA, IF, IHC (p), RNAi 宿主: 小鼠 Monoclonal 3H7 unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 1-495) antibodyRAD18 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 332-430) antibodyRAD18 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2G8C10 unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 216-495) antibodyRAD18 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 1-495) antibodyRAD18 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (Internal Region) antibodyRAD18 适用: 人, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 1-495) antibodyRAD18 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (Internal Region) antibodyRAD18 适用: 人, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 216-495) antibodyRAD18 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase RAD18 (RAD18) (Center) antibodyRAD18 适用: 人 IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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1) Western blotting: 1,000 fold dilution
2) Indirect immunofluorescence staining
3) Immunoprecipitation -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, 50 % glycerol
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储存液
- Azide free
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储存条件
- -20 °C
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: "Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination." in: The EMBO journal, Vol. 23, Issue 19, pp. 3886-96, (2004) (PubMed).
: "Differential regulation of Rad18 through Rad6-dependent mono- and polyubiquitination." in: The Journal of biological chemistry, Vol. 280, Issue 1, pp. 515-24, (2004) (PubMed).
: "Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 14, pp. 7927-32, (2000) (PubMed).
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: "Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination." in: The EMBO journal, Vol. 23, Issue 19, pp. 3886-96, (2004) (PubMed).
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- RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))
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别名
- Rad18
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背景
- The Rad6-Rad18 pair of genes plays a critical role in post-replication repair of damaged DNA. Rad6 protein functions as an E2 enzyme and Rad18 (495 aa, 56 kDa) as an ubiquitine ligase (E3) which ubiquitinates PCNA. Rad18 recruits translesion DNA polymerases to damaged DNA.
抗原
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