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NUP98 抗体 (GLFG Motif, N-Term)

This anti-NUP98 antibody is a 小鼠 单克隆 antibody detecting NUP98 in WB 和 IF. Suitable for 人, 小鼠, Saccharomyces cerevisiae, Schizosaccharomyces pombe 和 Tetrahymena. This Primary Antibody has been cited in 2+ publications.
产品编号 ABIN2452064
发货至: 中国

Quick Overview for NUP98 抗体 (GLFG Motif, N-Term) (ABIN2452064)

抗原

See all NUP98 抗体
NUP98 (Nucleoporin 98kDa (NUP98))

适用

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人, 小鼠, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Tetrahymena

宿主

  • 45
  • 11
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小鼠

克隆类型

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  • 15
单克隆

标记

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This NUP98 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF)

克隆位点

21A10
  • 抗原表位

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    GLFG Motif, N-Term

    交叉反应 (详细)

    Not tested in other species.

    纯化方法

    Purified

    过滤

    Sterile filtered

    免疫原

    Synthetic peptides containing conserved N-terminal sequence, GLFG, of Nup98 protein of Tetrahymena thermophila.

    亚型

    IgG1 kappa
  • 应用备注

    1) Western blotting: S. cerevisiae, S. pombe. Not suitable for human. Clone 13C2 is recommended for WB in human Tetrahymena and yeasts.
    2) Most suitable for Immunofluorescence staining / Immunocytochemistry for human, mouse, Tetrahymena, S. cerevisiae, S. pombe.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS, 50 % glycerol. Azide- and carrier protein-free.

    储存液

    Azide free

    储存条件

    -20 °C

    储存方法

    Upon arrival centrifuge briefly and store at -20 C.
  • Iwamoto, Asakawa, Ohtsuki, Osakada, Koujin, Hiraoka, Haraguchi: "Monoclonal antibodies recognize gly-leu-phe-gly repeat of nucleoporin nup98 of tetrahymena, yeasts, and humans." in: Monoclonal antibodies in immunodiagnosis and immunotherapy, Vol. 32, Issue 2, pp. 81-90, (2013) (PubMed).

    Iwamoto, Asakawa, Hiraoka, Haraguchi: "Nucleoporin Nup98: a gatekeeper in the eukaryotic kingdoms." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 15, Issue 7, pp. 661-9, (2010) (PubMed).

  • 抗原

    NUP98 (Nucleoporin 98kDa (NUP98))

    别名

    Nup98

    背景

    Nucleoporin 98 (Nup98) is a component of nuclear pore complex (NPC), which is a large protein assembly embedded in the nuclear envelope and highly conserved in eukaryotes. It is localized on both nuclear and cytoplasmic side of NPC. This protein contains glycine-leucine-phenylalanine- glycine (GLFG) amino acid repeats and plays a critical role in nuclear trafficking. Nup98 also plays a specific role in the RNA export. In addition, Nup98 plays roles in several important biological events such as gene expression, mitotic checkpoint, and pathogenesis Nup98 gene is fused to a variety of partner genes in human myeloid and T-cell malignancies via chromosomal translocation. In ciliates, a unicellular organism having two functionally distinct nuclei, GLFG-Nup98 is present in one of the nuclei and a distinct Nup98 ortholog is present in the other nucleus, and these different Nup98s participate in a nucleus-selective transport mechanism.

    UniProt

    P52948

    途径

    Stem Cell Maintenance, Protein targeting to Nucleus, SARS-CoV-2 Protein Interactome
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