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NUP153 抗体 (AA 610-1191)

Cited in 2+ publications. The 大鼠 单克隆 anti-NUP153 antibody (Clone R4C8) (ABIN2452062) specifically detects NUP153 in WB, ELISA, IF 和 ICC. The antibody is reactive with 大鼠 samples.
产品编号 ABIN2452062
发货至: 中国
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Quick Overview for NUP153 抗体 (AA 610-1191) (ABIN2452062)

抗原

See all NUP153 抗体
NUP153 (Nucleoporin 153kDa (NUP153))

适用

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大鼠

宿主

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大鼠

克隆类型

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单克隆

标记

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This NUP153 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

克隆位点

R4C8
  • 抗原表位

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    AA 610-1191

    特异性

    Specific to human, mouse, rat and monkey Nup153 proteins.

    交叉反应 (详细)

    Not tested for other species

    产品特性

    When injected into the nucleus,R4C8 accumulates into the nuclear pores of Hela cells. R4C8 works in immuno-cytochemistry very well.

    纯化方法

    Purified

    过滤

    Sterile filtered

    免疫原

    Recombinant GST-fused rat Nup153 (610-1191aa, Zn finger and FG repeats domain)

    亚型

    IgG2a kappa
  • 应用备注

    1. Western blotting: 160 kDa single band in Hela cell extract
    2. Immunocytochemistry
    3. ELISA
    Other applications have not been tested.

    Additional comments:
    When injected into the nucleus, R4C8 accumulates into the nuclear pores of Hela cells.
    R4C8 works in immuno-cytochemistry very well.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS, 50 % glycerol

    储存液

    Azide free

    储存条件

    -20 °C

    储存方法

    Upon receipt aliquot and store at -20 C
  • Lim, Fahrenkrog, Köser, Schwarz-Herion, Deng, Aebi: "Nanomechanical basis of selective gating by the nuclear pore complex." in: Science (New York, N.Y.), Vol. 318, Issue 5850, pp. 640-3, (2007) (PubMed).

    Ullman, Shah, Powers, Forbes: "The nucleoporin nup153 plays a critical role in multiple types of nuclear export." in: Molecular biology of the cell, Vol. 10, Issue 3, pp. 649-64, (1999) (PubMed).

  • 抗原

    NUP153 (Nucleoporin 153kDa (NUP153))

    别名

    Nup153

    背景

    The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nup (Nucleoporin) 153 is a large (153 kDa) O-linked glycoprotein which is a component of the basket structure located on the nucleoplasmic face of NPC. Nup153 plays a critical role in nuclear export of RNA and proteins. The antibody was purified from the serum-free cultured medium of the hybridoma under mild conditions by propriety chromatography processes.

    UniProt

    P49790

    途径

    Protein targeting to Nucleus
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