5-Methylcytosine 抗体 (Biotin)
(4 references)Quick Overview for 5-Methylcytosine 抗体 (Biotin) (ABIN2451913)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Purified
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过滤
- Sterile filtered
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免疫原
- 5-Methylcytosine conjugated to bovine serum albumin
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亚型
- IgM
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anti-5-Methylcytosine antibody
WB, FACS, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-5-Methylcytosine antibody适用: 人 MeDIP, IF, DB 宿主: 小鼠 Monoclonal A1 unconjugated
anti-5-Methylcytosine antibody (FITC)ICC, IF 宿主: 小鼠 Monoclonal 5MC-CD FITC
anti-5-Methylcytosine antibodyICC, IF 宿主: 小鼠 Monoclonal 5MC-CD unconjugated
anti-5-Methylcytosine antibodyWB, MeDIP, FACS, ICC, IF, DB, IHC, ELISA 宿主: 小鼠 Monoclonal 33D3 unconjugated
anti-5-Methylcytosine antibodyMeDIP, DB, MeDIP-Seq 宿主: 小鼠 Monoclonal A1 unconjugated
anti-5-Methylcytosine antibodyMeDIP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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1) Immunocytochemistry: ~50-100 fold dilution
2) Immuno-blotting detection of DNA with 5-methylcytosine on nitrocellulose: ~1000 fold dilution
Not tested with other application -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS with 50 % glycerol. Azide and carrier-protein free.
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储存液
- Azide free
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储存条件
- -20 °C
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储存方法
- Upon receipt aliquot and store at -20 C
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: "The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA." in: Nature, Vol. 450, Issue 7171, pp. 908-12, (2007) (PubMed).
: "A chloroplast-resident DNA methyltransferase is responsible for hypermethylation of chloroplast genes in Chlamydomonas maternal gametes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 9, pp. 5925-30, (2002) (PubMed).
: "Detection of heavy methylation in human repetitive DNA subsets by a monoclonal antibody against 5-methylcytosine." in: Biochimica et biophysica acta, Vol. 951, Issue 1, pp. 157-65, (1988) (PubMed).
: "Identification of 5-methylcytosine in DNA fragments immobilized on nitrocellulose paper." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, Issue 6, pp. 3581-5, (1980) (PubMed).
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: "The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA." in: Nature, Vol. 450, Issue 7171, pp. 908-12, (2007) (PubMed).
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- 5-Methylcytosine
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别名
- 5 Methylcytosine
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物质类
- Chemical
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背景
- DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently removed without changing the original DNA sequence. Therefore it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation results in addition of a methyl group to DNA - for example, to the number 5 carbon of the cytosine pyrimidine ring - which involves reduction in gene expression. In adult somatic tissues, DNA methylation typically occurs in a CpG dinucleotide context, non-CpG methylation is prevalent in embryonic stem cells. This hybridoma has been constructed by Prof. H. Sano.
抗原
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