CRB3 抗体 (full length)
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Quick Overview for CRB3 抗体 (full length) (ABIN2443902)
抗原
See all CRB3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- full length
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原理
- CRB3 Rat Monoclonal Antibody (14F9)
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特异性
- The antibody recognizes the full length CRB3 protein but not the extra cellular domain of CRB3. Recognize the overexpressed human form and the endogenous form in mouse.
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纯化方法
- Protein G purified
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免疫原
- Full length protein
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亚型
- IgG2c
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anti-Crumbs Homolog 3 (CRB3) (AA 1-120) antibody
CRB3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Crumbs Homolog 3 (CRB3) (AA 67-95) antibodyCRB3 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB36946 unconjugated
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应用备注
- WB 1:1000,IHC 1:100,IF 1:100
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 100 μL: WB 1:1000, IHC 1:100, IF 1:100. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized protein G purified in PBS pH 7.4
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- CRB3 (Crumbs Homolog 3 (CRB3))
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别名
- CRB3
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背景
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Crumbs homologue 3
CRB3 (Crumbs homologue 3) is an apical transmembrane protein member of the CRB protein family reported to be associated with tight junctions at the apical surface of epithelial cells and to play a role in apical membrane morphogenesis and tight junction regulation. The formation and maintenance of tight junctions is essential for the development of epithelial cell polarity. Two CRB3 isoforms have been described and CRB3 is homologous to the Crumb protein found in Drosophila. CRB3 contains a putative signal peptide in its first 20 amino acids, a putative extracellular site, and a transmembrane domain. The intracellular domain contains a PDZ-binding motif which is involved in the interactions of CRB3 with PARD6A and MPP5. Loss of CRB3 expression in immortal baby mouse kidney (iBMK) cells was reported to render these cells tumorigenic, suggesting an important role for CRB3 function in maintaining epithelial junction formation and apicobasal polarity required for contact-inhibited growth, and to suppress invasion and metastasis.
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UniProt
- Q9BUF7
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途径
- Notch Signaling, Cell-Cell Junction Organization
抗原
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