HGS 抗体 (full length)
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Quick Overview for HGS 抗体 (full length) (ABIN2443821)
抗原
See all HGS 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- full length
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原理
- Hrs (HGS) Mouse Monoclonal Antibody (19-5)
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特异性
- The antibody recognizes an epitope is located between the VHS and coiled-coil domain of the HRS protein
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纯化方法
- Protein G purified
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免疫原
- Full length hrs-2 fusion protein
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亚型
- IgG2b
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 50 μL: IHC: 1:300, ICC: 1:200, WB: 1:1000. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized protein G purified in PBS pH 7.4
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- HGS (Hepatocyte Growth Factor-Regulated tyrosine Kinase Substrate (HGS))
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别名
- Hrs (HGS)
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背景
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Hepatocyte growth factor-regulated tyrosine kinase substrate, HGS, Protein pp110
Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS, also known as HGS) is a mammalian protein predominantly localized on early endosomes. Hrs interacts with many proteins involved in membrane trafficking, such as eps15, SNX-1 and SNAP-25. Hrs is implicated in early endosome fusion and may play a role in cargo sorting/trafficking to early and late endosome by recruiting clathrin. Hrs is also involved in intracellular signal transduction mediated by cytokines and growth factors.
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UniProt
- O14964
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途径
- EGFR Signaling Pathway, CXCR4-mediated Signaling Events, Synaptic Vesicle Exocytosis, EGFR Downregulation
抗原
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