Nerve Growth Factor 抗体
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Quick Overview for Nerve Growth Factor 抗体 (ABIN2443811)
抗原
See all Nerve Growth Factor (NGF) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Nerve growth Factor (NGF) Rat Monoclonal Antibody (NGF30)
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特异性
- This antibody recognizes mouse NGF monomer protein (MW 13 kDa) by WB. It does not cross react with EGF, BSA, APO or insulin.
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纯化方法
- Protein G purified
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免疫原
- polymerized NGF
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亚型
- IgG2a
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应用备注
- WB 1:200
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 100 μL: WB 1:200. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized protein G purified in PBS pH 7.4
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- Nerve Growth Factor (NGF)
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背景
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Beta-nerve growth factor, Beta-NGF, NGF
Nerve growth factor (NGF) is a small secreted protein which induces the differentiation and survival of particular target neurons. It is perhaps the prototypical growth factor, in that it is one of the first to be described. NGF is critical for the survival and maintenance of sympathetic and sensory neurons. NGF is released from the target cells, binds to and activates its high affinity receptor (TrkA), and is internalized into the responsive neuron. There is some data that shows that NGF can be transported from the axon tip to soma. One of the most important molecules in the nervous system, nerve growth factor and its other neurotrophin family members control the development of the nervous system in the embryo and the maintenance of nervous tissue and neural transmission in the adult.
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UniProt
- P01139
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途径
- Regulation of Cell Size
抗原
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