DYKDDDDK Tag 抗体 (C-Term, N-Term)
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Quick Overview for DYKDDDDK Tag 抗体 (C-Term, N-Term) (ABIN2443789)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term, N-Term
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原理
- FLAG Tag Mouse Monoclonal Antibody (FG4R)
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序列
- DYKDDDDK
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特异性
- The antibody recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins.
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纯化方法
- Protein G purified
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免疫原
- The antibody was raised against DYKDDDDK (FLAG) synthetic peptide conjugated to KLH.
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亚型
- IgG2b
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anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal FG4R unconjugated
anti-DYKDDDDK Tag (Cleavage Site) antibodyWB, ELISA, IP, ICC 宿主: 兔 Monoclonal BLRE00G unconjugated Recombinant Antibody
anti-DYKDDDDK Tag antibody适用: 人 WB 宿主: 兔 Polyclonal RB0493 unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 5A8E5 unconjugated
anti-DYKDDDDK Tag antibody适用: 标记 WB, ELISA, IP, FACS, IHC, ICC 宿主: 小鼠 Monoclonal 2F11G1 unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP, ICC 宿主: 山羊 Polyclonal unconjugated
anti-DYKDDDDK Tag antibody (HRP)WB, ELISA, IP 宿主: 兔 Polyclonal HRP
anti-DYKDDDDK Tag antibodyWB, ELISA, FACS, IHC 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated
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应用备注
- WB 1:1000,IHC 1:500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 100 μL: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized protein G purified in PBS pH 7.4
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- DYKDDDDK Tag
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别名
- FLAG Tag
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物质类
- Tag
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背景
- Epitope tags have application in the labeling, isolation and detection of proteins using immunoblotting, Immunoprecipitation and immunostaining techniques. Epitope tags can be used, by affinity chromatography, to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. Due its small size the protein's biochemical properties seem not to be affected by the tag protein. FLAG-tag, or FLAG octapeptide, a polypeptide consisting of eight amino acids (DYKDDDDK) can be added to a protein using recombinant DNA technology, therefore, the tag can be detected with the fused protein in many different assays that require recognition by an antibody.
抗原
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