The antibody MEM-E/08 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. The published results revealed that antibody is remarkably specific for HLA-E, only with weak cross-reactivity with following classical MHC Class I molecules (MHC Class Ia): HLA-A24, -B7, -B27, -B51, -B54, -C7.
交叉反应 (详细)
Species reactivity (tested):Human
产品特性
Synonyms: HLA-6.2, HLAE, MHC class I antigen E, HLA class I histocompatibility antigen, alpha chain E
纯化方法
Protein A-affinity chromatography
纯度
> 95 % by SDS-PAGE
免疫原
Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide. Remarks: Gene Locus: 6p21. 3
Flow Cytometry. Positive control: human cell line LCL 721.221 (HLA-E transfected). Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
有效期
12 months
抗原
HLA-E
(HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))
EA1.2 antibody, EA2.1 antibody, HLA-6.2 antibody, MHC antibody, QA1 antibody, major histocompatibility complex, class I, E antibody, HLA-E antibody, PATR-E antibody
背景
HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on many types of the human cells.Synonyms: HLA class I histocompatibility antigen, HLA-6.2, HLAE, MHC class I antigen E, alpha chain E