ACRV1 抗体
Quick Overview for ACRV1 抗体 (ABIN238420)
抗原
See all ACRV1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The antibody Hs-14 reacts with a 220 kDa testis-specific intra-acrosomal protein associated with the membranes of the acrosomal vesicle.
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产品特性
- Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein
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纯化方法
- Precipitation methods and size-exclusion chromatography
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid,10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% aceticacid and subsequently used for immunization.
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亚型
- IgM
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anti-Acrosomal Vesicle Protein 1 (ACRV1) (AA 22-265) antibody
ACRV1 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 5E3D6 unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (AA 22-265) antibodyACRV1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2D10D5 unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (AA 22-265) antibodyACRV1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (AA 22-265) antibodyACRV1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (AA 1-265) antibodyACRV1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (N-Term) antibodyACRV1 适用: 人, 豚鼠, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) antibodyACRV1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (N-Term) antibodyACRV1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (N-Term) antibodyACRV1 适用: 人, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry. Western Blotting. Immunocytochemistry: 10 μg/mL, Staining technique: Membrane permeabilization(acetone) is essential. Application note: The antibody Hs-14 is designed for quantitativeimmunofluorescence analysis of pathological sperms (excellent tool for laboratories ofassisted reproduction when optimal method of fertilization is sought).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
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Store the antibody at 2 - 8 °C. DO NOT FREEZE!
Shelf life: one year from despatch. -
有效期
- 12 months
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- ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))
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别名
- ACRV1
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背景
- One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.Synonyms: Acrosomal protein SP-10, Acrosomal vesicle protein 1, Intra-acrosomal Sperm Protein, SP10, SPACA2
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基因ID
- 56
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UniProt
- P26436
抗原
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