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ACRV1 抗体

ACRV1 适用: 犬 WB, ICC 宿主: 小鼠 Monoclonal Ds-2 unconjugated
产品编号 ABIN93928
发货至: 中国
  • 抗原 See all ACRV1 抗体
    ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))
    适用
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    宿主
    • 26
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    小鼠
    克隆类型
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    单克隆
    标记
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    This ACRV1 antibody is un-conjugated
    应用范围
    • 32
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    Western Blotting (WB), Immunocytochemistry (ICC)
    特异性
    The mouse monoclonal antibody Ds-2 reacts with canine intra-acrosomal sperm protein (92 and 42 kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.
    交叉反应 (详细)
    Canine (Dog)
    纯化方法
    Purified by protein-A affinity chromatography.
    纯度
    > 95 % (by SDS-PAGE)
    免疫原
    Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.
    克隆位点
    Ds-2
    亚型
    IgG
    Top Product
    Discover our top product ACRV1 Primary Antibody
  • 应用备注
    Immunocytochemistry: Membrane permeabilization (acetone) is essential.
    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze.
    储存条件
    4 °C
    储存方法
    Store at 2-8°C. Do not freeze.
  • Geussová, P?knicová, Capková, Kaláb, Moos, Philimonenko, Hozák: "Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction." in: Andrologia, Vol. 29, Issue 5, pp. 261-8, (1997) (PubMed).

  • 抗原
    ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))
    别名
    Intra-Acrosomal Protein (ACRV1 产品)
    背景
    One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,IAP Can
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