STIM1 抗体 (C-Term, Cytoplasmic Domain)
Quick Overview for STIM1 抗体 (C-Term, Cytoplasmic Domain) (ABIN238397)
抗原
See all STIM1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term, Cytoplasmic Domain
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特异性
- The antibody CDN3H4 reacts with human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.
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交叉反应 （详细）
- Species reactivity (tested):Human, Mouse, Rat.
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产品特性
- Synonyms: Stromal interaction molecule 1
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纯化方法
- Protein-A affinity chromatography
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免疫原
- Synthetized peptide (C-terminal cytoplasmic part of STIM1)
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亚型
- IgG1
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anti-Stromal Interaction Molecule 1 (STIM1) (AA 24-685) antibody
STIM1 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 5A2 unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (AA 1-211) antibodySTIM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (C-Term) antibodySTIM1 适用: 人, 大鼠, 小鼠 WB, IP, IHC (p), ICC 宿主: 小鼠 Monoclonal CDN3H4 unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (AA 24-686) antibodySTIM1 适用: 人 WB, IHC (p), IF, EIA 宿主: 小鼠 Monoclonal 5A2 unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (AA 24-686) antibodySTIM1 适用: 人 WB, ELISA, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 5A2 unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (AA 56-69), (Extracellular) antibodyKO Validated STIM1 适用: 人 WB, IHC, IP, IF, FACS, IC, LCI 宿主: 兔 Polyclonal unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (AA 509-538), (C-Term) antibodySTIM1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB19222 unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (Middle Region) antibodySTIM1 适用: 人, 大鼠, 小鼠, 犬, Cow, 豚鼠, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (AA 509-538) antibodySTIM1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (C-Term) antibodySTIM1 适用: 人, 大鼠, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunoprecipitation. Western Blotting (reducing and non-reducing conditions): Recommended dilution: 1 μg/mLPositive control: RBL rat basophilic leukemia cell lineSample preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing/reducing Laemmli SDS-PAGE sample buffer. Immunocytochemistry: Staining technique: methanol-aceton fixation. Positive control: HeLa human cervix carcinoma cell line. Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4, 15 mM Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- STIM1 (Stromal Interaction Molecule 1 (STIM1))
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别名
- STIM1 / GOK
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背景
- STIM1 (stromal interacting molecule, also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.Synonyms: Stromal interaction molecule 1
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基因ID
- 6786
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UniProt
- Q13586
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途径
- TCR Signaling, BCR Signaling
抗原
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