SMAD4 抗体 (C-Term)
Quick Overview for SMAD4 抗体 (C-Term) (ABIN233840)
抗原
See all SMAD4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-a41
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Supplier
- Rockland
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原理
- SMAD4 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human SMAD4 protein.
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产品特性
- Synonyms: rabbit anti-SMAD4 antibody, SMAD-4, SMAD 4, mothers against decapentaplegic homolog 4 antibody, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4 antibody, SMAD family member 4, DPC 4, DPC4, MADH4, MADH 4
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human SMAD4 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-SMAD Family Member 4 (SMAD4) (N-Term) antibody
SMAD4 适用: 人 WB, IP, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 1-552) antibodySMAD4 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 3E2 unconjugated
anti-SMAD Family Member 4 (SMAD4) antibodyKO Validated SMAD4 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) antibodySMAD4 适用: 人, 小鼠 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 4G1C6 unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 400-428) antibodySMAD4 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 27-195) antibodySMAD4 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 255-284) antibodySMAD4 适用: 人, 小鼠 WB, IF 宿主: 兔 Polyclonal RB14714 unconjugated
anti-SMAD Family Member 4 (SMAD4) (pThr277) antibodySMAD4 适用: 人 ELISA, IHC, IF, DB 宿主: 兔 Polyclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) antibodySMAD4 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 3A1 unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 9-231) antibodySMAD4 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 60 kDa in size corresponding to SMAD4 protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:15,000 - 1:60,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.2 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- SMAD4 (SMAD Family Member 4 (SMAD4))
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别名
- SMAD4
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. SMAD4 (also known as Mothers against decapentaplegic homolog 4, Mothers against DPP homolog 4, deletion target in pancreatic carcinoma 4 and hSMAD4) is a common mediator of signal transduction by TGF-b (transforming growth factor), but is also involved in cancer development and metastases as a tumor suppressor. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may form trimers with receptor-regulated SMAD (R-SMAD) and interacts with ATF2, COPS5, DACH1, MSG1, SKI and TRIM33. In the absence of ligand SMAD4 is found in the cytoplasm, but when complexed with R-SMAD, translocates to the nucleus. Defects in SMAD4 are a cause of pancreatic carcinoma and juvenile polyposis syndrome (JPS), a syndrome in which patients are at risk for developing gastrointestinal cancers.
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基因ID
- 4089, 13603414
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UniProt
- Q13485
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途径
- Cell Division Cycle, Chromatin Binding, Autophagy
抗原
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