(1 reference)
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- 抗原 See all ASAP3 抗体
- ASAP3 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 3 (ASAP3))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ASAP3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 200-401-990
- Supplier
- Rockland
- 原理
- UPLC1/ASAP3 Antibody
- 交叉反应 （详细）
- This protein A purified antibody is directed against human UPLC1/ASAP3 protein.
- 产品特性
- Synonyms: rabbit anti-UPLC1 Antibody, rabbit anti-ASAP3 Antibody, UPLC1/ASAP3 Antibody, Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3, FLJ20199 antibody, Gm140 antibody, Development and differentiation-enhancing factor-like 1, Protein up-regulated in liver cancer 1, DDEFL1
- 纯化方法
- The product was purified from monospecific antiserum by protein A chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant human UPLC1/ASAP3 protein.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
- Top Product
- Discover our top product ASAP3 Primary Antibody
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anti-ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 3 (ASAP3) (AA 451-700) antibody
ASAP3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 3 (ASAP3) (AA 26-54), (N-Term) antibodyASAP3 适用: 人, Hamster WB, IHC (p) 宿主: 兔 Polyclonal RB25488 unconjugated
anti-ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 3 (ASAP3) (AA 26-54), (N-Term) antibodyASAP3 适用: 人, Hamster WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 3 (ASAP3) (AA 693-710) antibodyASAP3 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 3 (ASAP3) (AA 35-84) antibodyASAP3 适用: 人, Cow, 豚鼠, 马, 斑马鱼, Bat, 猴, Pig, 兔 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: This protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 100 kDa in size corresponding to UPLC1/ASAP3 protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:200 - 1:2,000
ELISA Dilution: 1:1,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store UPLC1 / ASAP3 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Loss of ASAP3 destabilizes cytoskeletal protein ACTG1 to suppress cancer cell migration." in: Molecular medicine reports, Vol. 9, Issue 2, pp. 387-94, (2014) (PubMed).
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: "Loss of ASAP3 destabilizes cytoskeletal protein ACTG1 to suppress cancer cell migration." in: Molecular medicine reports, Vol. 9, Issue 2, pp. 387-94, (2014) (PubMed).
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- 抗原
- ASAP3 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 3 (ASAP3))
- 别名
- ASAP3 (ASAP3 产品)
- 背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-UPLC1 (up-regulated in liver cancer 1) / ASAP3 Antibody, also named DDEFL1 (development and differentiation-enhancing factor-like 1) or ASAP3, is a member of the AZAP family of proteins. These proteins catalyze the hydrolysis of GTP bound to ADP-ribosylation factor (Arf) proteins, thereby causing Arf inactivation. For this reason, the ASAPs are generally called ArfGAPs. The activity of ArfGAPs is dependent on the presence of phosphoinositides and is implicated in cellular processes such as membrane trafficking and remodeling of the actin cytoskeleton. ASAP3 has been found to be up-regulated in 80 % of the hepatocellular carcinomas examined. Initial biochemical characterization reveals that ASAP3 shows class-specific GAP activity on Arf proteins, preferring Arf5 over Arf1 and Arf6. ASAP3 antibody has beed developed through the NCI antibody collaboration program and is ideal for Cancer and Signal Transduction research.
- 基因ID
- 55616, 19071867
- UniProt
- Q8TDY4
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