Mesothelin 抗体
(2 references)
Quick Overview for Mesothelin 抗体 (ABIN233752)
抗原
See all Mesothelin (MSLN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-a87
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Supplier
- Rockland
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原理
- Mesothelin Antibody
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交叉反应 （详细）
- This antibody is directed against human mesothelin protein.
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产品特性
- Synonyms: mouse anti-Mesothelin Antibody, Mesothelian, MN, MB, Pre-pro-megakaryocyte-potentiating factor, CAK1 antigen
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纯化方法
- This product was purified from tissue culture supernatant fluid by Protein A chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG2a
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anti-Mesothelin (MSLN) (AA 273-407) antibody
MSLN 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal MSLN-2131 unconjugated
anti-Mesothelin (MSLN) (AA 101-200) antibodyMSLN 适用: 人 ELISA, WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Mesothelin (MSLN) (AA 273-407) antibodyMSLN 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal unconjugated
anti-Mesothelin (MSLN) (AA 37-286) antibodyMSLN 适用: 人, 猴 ELISA, WB, FACS 宿主: 小鼠 Monoclonal 5C11A4 unconjugated
anti-Mesothelin (MSLN) (AA 37-598) antibodyMSLN 适用: 人 ELISA, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Mesothelin (MSLN) (AA 296-580) antibodyMSLN 适用: 人 ELISA, FACS 宿主: 兔 Monoclonal DM71 unconjugated Recombinant Antibody
anti-Mesothelin (MSLN) (AA 296-606) antibodyMSLN 适用: 人, 大鼠 ELISA, WB 宿主: 小鼠 Monoclonal 7E6A6 unconjugated
anti-Mesothelin (MSLN) (AA 37-286) antibodyMSLN 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 4H12F8 unconjugated
anti-Mesothelin (MSLN) (AA 1-62) antibodyMSLN 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mesothelin (MSLN) antibodyMSLN 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry Dilution: 1:200
Immunohistochemistry Dilution: 1:100
Application Note: This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For Anti-mesothelin immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1 % BSA in PBS for 30 min at 23° C. Antibodies are diluted in 1 % BSA and reacted with tissue for 60 min at room temperature.
Western Blot Dilution: 1:1,000
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Mesothelin expression in human lung cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 5, pp. 1571-5, (2007) (PubMed).
: "New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 11, Issue 16, pp. 5840-6, (2005) (PubMed).
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: "Mesothelin expression in human lung cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 5, pp. 1571-5, (2007) (PubMed).
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- Mesothelin (MSLN)
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别名
- MSLN
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背景
- Background: Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol-anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71- kDa precursor protein that is processed to a 40- kDa glycosylphosphatidylinositol-anchored protein that composes the mature portion and an NH2 terminal 31- kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.
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基因ID
- 10232, 53988378
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UniProt
- Q13421
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途径
- EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process
抗原
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