MLANA 抗体
Quick Overview for MLANA 抗体 (ABIN228816)
抗原
See all MLANA 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes MART-1 at 18kD. Labels melanomas and other tumors showing melanocytic differentiation. Does not label tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Species cross-reactivity: Human and equine. Does not cross-react with mouse or rat.
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纯化方法
- Protein A purified
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免疫原
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Recombinant hMART-1 protein. MW: 18kD. Cellular Localization: Cytoplasmic.
Type of Immunogen: Recombinant protein -
亚型
- IgG2b kappa
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应用备注
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Approved: Flo, IHC, IHC-P, IP, WB (0.5 - 1 μg/mL)
Usage: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Denatured verified): 2 μg/mg protein lysate. (Use Protein A). Western Blot: 0.5-1 μg/mL for 2 hours at RT. Immunohistochemistry (formalin/paraffin): 1-2 μg/mL for 30 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: CaC1 melanoma cells and melanomas. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.1 % BSA, 0.05 % sodium azide, 40 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- MLANA (Melan A (MLANA))
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别名
- MLANA / Melan-A / MART-1
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背景
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Name/Gene ID: MLANA
Synonyms: MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1 -
基因ID
- 2315
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UniProt
- Q16655
抗原
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