CTNNB1 抗体 (pTyr142)
Quick Overview for CTNNB1 抗体 (pTyr142) (ABIN227283)
抗原
See all CTNNB1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr142
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特异性
- Recognizes a 92kD protein corresponding to the molecular mass of b-Catenin on SDS-PAGE immunoblots of Hct116 src transformed cells treated with pervanadate, but not in control cells. Similar results were observed in other pervanadate-treated cell lines, such as A431 and human endothelial cells. Species sequence homology: mouse and rat. Sequence homology: one amino acid difference from a sequence around tyrosine 133 of human g-Catenin.
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide corresponding to amino acid residues around tyrosine 142 of human b-Catenin (KLH).
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: ELISA (1:2000), WB (1:500)
Usage: Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols. Western Blot: 1:500. Membrane was incubated with diluted antibody in 5 % non-fat milk, PBS, 0.04 % Tween 20 for 1 hour at RT. ELISA: 1:2000. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, 1 mg/mL BSA, 0.05 % sodium azide, 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
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别名
- CTNNB1 / Beta Catenin
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背景
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Name/Gene ID: CTNNB1
Family: Catenin
Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin -
基因ID
- 1499
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UniProt
- P35222
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途径
- WNT signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
抗原
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