Caspase 3 抗体 (N-Term)
Quick Overview for Caspase 3 抗体 (N-Term) (ABIN219228)
抗原
See all Caspase 3 (CASP3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- Recognizes cleaved human Caspase 3. Detects endogenous levels of the large fragment (17/19kD) of activated caspase-3 resulting from cleavage adjacent to Asp175. Does not recognize full length caspase-3 or other cleaved caspases. Species cross-reactivity: mouse, bovine, and rat.
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide corresponding residues adjacent to Asp175 in N-terminus of human caspase-3 (KLH coupled).
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: ELISA, Flo (1:200), ICC, IF, IHC, IHC-Fr (1:200), WB (1:1000)
Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Recommend Dilution: Western Blot: 1:1000. Immunohistochemistry (Frozen): 1:200. Immunocytochemistry (IF):1:200. Flow Cytometry: 1:200.
Not recommended for: IHC-P -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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储存液
- Without preservative
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注意事项
- avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Caspase 3 (CASP3)
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别名
- CASP3 / Caspase 3
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背景
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Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease
Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama -
基因ID
- 836
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UniProt
- P42574
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途径
- Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
抗原
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