For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/mL), IL-1 (100 U/mL) or LPS (1 μg/mL) in tissue culture medium at 37° C.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
有效期
12 months
抗原
ICAM1
(Intercellular Adhesion Molecule 1 (ICAM1))
别名
Icam-1, CD54
背景
The antibody reacts with both intact human ICAM-1, CD54 and with soluble human ICAM-1. The antibody reacts also with ICAM-1 present on chimpanzee, rhesus monkey, cynomolgus monkey and baboon tissues.