Nitrotyrosine 抗体
(5 references)Quick Overview for Nitrotyrosine 抗体 (ABIN2192139)
抗原
See all Nitrotyrosine 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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交叉反应 （详细）
- Cross reactivity: Phosphotyrosine : No, Chlorotyrosine : No
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过滤
- 0.2 μm filtered
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亚型
- IgG2b
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anti-Nitrotyrosine antibody
WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 unconjugated
anti-Nitrotyrosine antibody (HRP)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 HRP
anti-Nitrotyrosine antibody (FITC)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 FITC
anti-Nitrotyrosine antibody (Biotin)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Biotin
anti-Nitrotyrosine antibody (PE)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 PE
anti-Nitrotyrosine antibody (PerCP)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 PerCP
anti-Nitrotyrosine antibody (APC)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 APC
anti-Nitrotyrosine antibody (Atto 594)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Atto 594
anti-Nitrotyrosine antibody (Atto 488)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Atto 488
anti-Nitrotyrosine antibody (Atto 390)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Atto 390
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应用备注
- For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive W: mouse kidney lysate, mouse optic nerve, retina , spinal cord and brain lysates, rat aorta lysate control P: human lung tissue
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限制
- 仅限研究用
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缓冲液
- PBS, containing 0.1 % bovine serum albumin
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储存条件
- 4 °C
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储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
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: "Tempol ameliorates murine viral encephalomyelitis by preserving the blood-brain barrier, reducing viral load, and lessening inflammation." in: Free radical biology & medicine, Vol. 48, Issue 5, pp. 704-12, (2010) (PubMed).
: "Protein nitration and poly-ADP-ribosylation in brain after rapid exsanguination cardiac arrest in a rat model of emergency preservation and resuscitation." in: Resuscitation, Vol. 79, Issue 2, pp. 301-10, (2008) (PubMed).
: "Protein nitration in a mouse model of familial amyotrophic lateral sclerosis: possible multifunctional role in the pathogenesis." in: The Journal of biological chemistry, Vol. 280, Issue 16, pp. 16295-304, (2005) (PubMed).
: "Local and peripheral cytokine response and CagA status of Helicobacter pylori-positive patients with duodenal ulcer." in: European cytokine network, Vol. 14, Issue 3, pp. 143-8, (2003) (PubMed).
: "Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich ELISA." in: Free radical biology & medicine, Vol. 25, Issue 8, pp. 953-63, (1999) (PubMed).
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: "Tempol ameliorates murine viral encephalomyelitis by preserving the blood-brain barrier, reducing viral load, and lessening inflammation." in: Free radical biology & medicine, Vol. 48, Issue 5, pp. 704-12, (2010) (PubMed).
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- Nitrotyrosine
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物质类
- Chemical
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背景
- The monoclonal antibody HM.11 recognizes modified amino acid nitrotyrosine in all different species. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Inflammation is characterized by increased nitric oxide (NO) production. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, Amyotrophic Lateral Sclerosis (ALS) and Multiple Sclerosis (MS). Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo and consequently as a marker of NO-mediated tissue damage. The monoclonal antibody HM.11 recognizes nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. Immunogen Nitrated KLH
抗原
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