(2 references)-
- 抗原 See all PRTN3 抗体
- PRTN3 (Proteinase 3 (PRTN3))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This PRTN3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA)
- 过滤
- 0.2 μm filtered
- 克隆位点
- PR3G-2
- Top Product
- Discover our top product PRTN3 Primary Antibody
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anti-Proteinase 3 (PRTN3) (AA 71-247) antibody
PRTN3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Proteinase 3 (PRTN3) (AA 88-98) antibodyVerified PRTN3 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Proteinase 3 (PRTN3) (AA 24-249) antibodyPRTN3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Proteinase 3 (PRTN3) (AA 1-170) antibodyPRTN3 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Proteinase 3 (PRTN3) (AA 228-256), (C-Term) antibodyPRTN3 适用: 人 WB 宿主: 兔 Polyclonal RB40945 unconjugated
anti-Proteinase 3 (PRTN3) (AA 1-256) antibodyPRTN3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Proteinase 3 (PRTN3) (AA 1-256) antibodyPRTN3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Proteinase 3 (PRTN3) antibodyPRTN3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B4 unconjugated
anti-Proteinase 3 (PRTN3) (AA 139-248) antibodyPRTN3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Proteinase 3 (PRTN3) (AA 76-125) antibodyPRTN3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
- 限制
- 仅限研究用
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- 缓冲液
- PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
- 有效期
- 12 months
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: "Proteinase-3 induces procaspase-3 activation in the absence of apoptosis: potential role of this compartmentalized activation of membrane-associated procaspase-3 in neutrophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 10, pp. 6381-90, (2005) (PubMed).
: "Characterization of monoclonal antibodies to proteinase 3 (PR3) as candidate tools for epitope mapping of human anti-PR3 autoantibodies." in: Clinical and experimental immunology, Vol. 118, Issue 3, pp. 487-96, (2000) (PubMed).
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: "Proteinase-3 induces procaspase-3 activation in the absence of apoptosis: potential role of this compartmentalized activation of membrane-associated procaspase-3 in neutrophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 10, pp. 6381-90, (2005) (PubMed).
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- 抗原
- PRTN3 (Proteinase 3 (PRTN3))
- 别名
- Proteinase 3 (PRTN3 产品)
- 别名
- ela2 antibody, MGC132250 antibody, ACPA antibody, AGP7 antibody, C-ANCA antibody, CANCA antibody, MBN antibody, MBT antibody, NP-4 antibody, NP4 antibody, P29 antibody, PR-3 antibody, PR3 antibody, mPR3 antibody, proteinase 3 L homeolog antibody, proteinase 3 antibody, prtn3.L antibody, PRTN3 antibody, Prtn3 antibody
- 背景
- Monoclonal antibody PR3G-2 reacts with human proteinase 3 (PR3), a 30 kDa protein. PR3 is a major antigen recognized by autoantibodies directed against cytoplasmic proteins of neutrophilic granulocytes and monocytes (called anti-neutrophil cytoplasmic autoantibodies (ANCA)). ANCA are able to activate primed neutrophils to produce oxygen radicals and release lytic enzymes, including PR3. Proteinase 3 (PR3) was identified as the target antigen of ANCA in Wegener's granulomatosis (WG). ANCA directed against PR3 (PR3-ANCA) can interfere with the binding of PR3 to its physiological inhibitor alpha1- antitrypsin (alpha1-AT) and with the proteolytic activity of PR3. At the site of inflammation, PR3 can cleave the PR3-ANCA complex between these inhibiting ANCA and PR3 itself, leaving active PR3. Autoantibodies to PR3 are potent activators of the 5-lipoxygenase pathway in primed human neutrophils. Extracellular free arachidonic acid, as present at an inflammatory focus, synergizes with such autoantibodies to evoke full-blown lipid mediator generation, granule secretion and respiratory burst. Proteinase 3 (PR3) is a neutral serine proteinase, which is localized in the azurophilic granules of neutrophils and in granules of monocytes and can be detected in the membrane of secretory vesicles. PR3 degrades a number of extracellular matrix proteins such as elastin and inactivates human C1 inhibitor. Membrane-associated PR3 is also able to activate caspase-3 without triggering apoptosis of neutrophils, which is possibly a neutrophil survival mechanism. In addition, PR3 is involved in myeloid differentiation and is, therefore, also called myeloblastin. The monoclonal antibody PR3-G2 was produced by immunization of mice with a crude granule extract.
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