This anti-Interferon gamma antibody is a 小鼠 单克隆 antibody detecting Interferon gamma in IP, Neut, IHC (fro) 和 IA. Suitable for 人. This Primary Antibody has been cited in 4+ publications.
For immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity in vitro dilutions have to be made according to the amount of IFN-gamma to be inactivated. One neutralizing unit of anti-human gamma interferon is defined as the amount of antibody sufficient for neutralizing one unit human gamma interferon (ref.: NIH standard Gg23- 901-530).
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute the vial by injection of 1 mLdistilled or de-ionized water (Caution: vial is under vacuum).
缓冲液
Lyophilized product in PBS, containing 300 μg antibody.
注意事项
Repeated freeze and thaw cycles will cause loss of activity. Under recommended storage conditions, product is stable for one year.
储存条件
4 °C
储存方法
Lyophilized product should be stored at 4 °C Store stock solution in aliquots at -20 °C Repeated freeze and thaw cycles will cause loss of activity. Under recommended storage conditions, product is stable for one year.
有效期
12 months
Billiau: "Gamma-interferon: the match that lights the fire?" in: Immunology today, Vol. 9, Issue 2, pp. 37-40, (1989) (PubMed).
Jacob, van der Meide, McDevitt: "In vivo treatment of (NZB X NZW)F1 lupus-like nephritis with monoclonal antibody to gamma interferon." in: The Journal of experimental medicine, Vol. 166, Issue 3, pp. 798-803, (1987) (PubMed).
Van der Meide, Dubbeld, Schellekens: "Monoclonal antibodies to human immune interferon and their use in a sensitive solid-phase ELISA." in: Journal of immunological methods, Vol. 79, Issue 2, pp. 293-305, (1985) (PubMed).
Armstrong: "Semi-micro, dye-binding assay for rabbit interferon." in: Applied microbiology, Vol. 21, Issue 4, pp. 723-5, (1971) (PubMed).
抗原
Interferon gamma (IFNG)
别名
gamma Interferon
背景
Monoclonal antibody binds and neutralizes both natural and recombinant human gamma Interferon. Cross reactivity with other cytokines has not been found. The antibody does not react with rodent interferons. Possible applications include the purification of human gamma Interferon and in vitro neutralisation. In general, monoclonal antibodies to gamma Interferon are able to inhibit Schwartzman reactions and in the murine system appear to protect NZB mice against spontaneous development of autoimmune disease.