CDK2 抗体 (AA 201-298)
(2 references)-
- 抗原 See all CDK2 抗体
- CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
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抗原表位
- AA 201-298
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CDK2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 交叉反应
- 人, 小鼠
- 预测反应
- Rat,Cow,Pig,Horse,Rabbit
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human CDK2
- 亚型
- IgG
- Top Product
- Discover our top product CDK2 Primary Antibody
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anti-Cyclin-Dependent Kinase 2 (CDK2) antibody
CDK2 适用: 人, 小鼠 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1A6 unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) (AA 211-298) antibodyCDK2 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 2E8 unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) antibodyCDK2 适用: 人, 猴 WB, FACS, IF 宿主: 小鼠 Monoclonal 4B4 unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) (AA 197-295) antibodyCDK2 适用: 人, 小鼠 WB, IHC, ELISA, FACS, IF 宿主: 小鼠 Monoclonal 1A6 unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) antibodyCDK2 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) (AA 1-298) antibodyCDK2 适用: 人 WB, IHC, ELISA, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) antibodyCDK2 适用: 人, 猴 WB, FACS, IF 宿主: 小鼠 Monoclonal 1C4 unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) (AA 81-298) antibodyCDK2 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) (AA 94-198) antibodyCDK2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) (AA 93-211) antibodyCDK2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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: "RBP-J-interacting and tubulin-associated protein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma by activating the p53-Fbxw7 pathway." in: Biochemical and biophysical research communications, Vol. 454, Issue 1, pp. 71-7, (2015) (PubMed).
: "CDK2-AP1 inhibits growth of breast cancer cells by regulating cell cycle and increasing docetaxel sensitivity in vivo and in vitro." in: Cancer cell international, Vol. 14, Issue 1, pp. 130, (2014) (PubMed).
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: "RBP-J-interacting and tubulin-associated protein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma by activating the p53-Fbxw7 pathway." in: Biochemical and biophysical research communications, Vol. 454, Issue 1, pp. 71-7, (2015) (PubMed).
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- 抗原
- CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
- 别名
- CDK2 (CDK2 产品)
- 别名
- p33(CDK2) antibody, A630093N05Rik antibody, wu:fa10c02 antibody, zgc:56598 antibody, zgc:77684 antibody, eg1 antibody, CDKN2 antibody, cdk2L antibody, cyclin dependent kinase 2 antibody, cyclin-dependent kinase 2 antibody, cyclin-dependent kinase 2 S homeolog antibody, CDK2 antibody, Cdk2 antibody, cdk2 antibody, cdk2.S antibody
- 背景
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Synonyms: CDKN2, p33(CDK2), Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinase, CDK2
Background: Serine/threonine-protein kinase involved in the control of the cell cycle, essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression, controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2, activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis, regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication.
- 基因ID
- 1017
- UniProt
- P24941
- 途径
- PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
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