TCP1 alpha/CCTA 抗体 (C-Term)
Quick Overview for TCP1 alpha/CCTA 抗体 (C-Term) (ABIN215497)
抗原
See all TCP1 alpha/CCTA (TCP1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- This affinity purified antibody recognizes an epitope in the C-terminus of mouse TCP-1a (465-AKLRA) and will detect an ~60kD protein, corresponding to the apparent molecular mass of TCP-1alpha on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, guinea pig, hamster, monkey, porcine and rabbit origins, as well as Saccharomyces cerevisiae (weakly, consistent with the epitope sequence being AKLRS), Drosophila, Schizosaccharomyces pombe and C. elegans. In C. elegans CTA-191 reacts with TCP-1a and another CCT subunit. In plants, CTA-191 recognizes TCP-1 of Pisum sativum and the sequence of Arabidopsis thalania TCP-1a over the region of the epitope AKLRA. It has also been shown that CTA-191 reacts with a subunit of a specialized chaperonin which folds phytochrome. Cross reactivity with human Hsp60 has been observed with this antibody in immunoblot analysis.
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纯化方法
- Protein G purified
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免疫原
- A purified recombinant mouse TCP-1alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp-1b gene expressed in E. coli.
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亚型
- IgG2a
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应用备注
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Approved: ICC (5 μg/mL), IP (12.5 μg/mL), WB
Usage: Western Blot (Colorimetric): 1 μg/mL. Immunoprecipitation: 12.5 μg/mL 5 μg/mL. Immunocytochemistry: 5 μg/mL. Positive control: HeLa Heat Shocked Cell Lysate. -
说明
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Target Species of Antibody: Rat
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.2, 0.1 mM PMSF, in 50 % glycerol.
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储存液
- PMSF
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))
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别名
- TCP1
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背景
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Name/Gene ID: TCP1
Synonyms: TCP1, CCTA, CCT-alpha, D6S230E, T-complex 1, TCP-1-alpha, Tailless complex polypeptide 1, CCT1 -
基因ID
- 6950
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UniProt
- P17987
抗原
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