GLI3 抗体 (AA 41-57)
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北京 101111
Quick Overview for GLI3 抗体 (AA 41-57) (ABIN214604)
抗原
See all GLI3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 41-57
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特异性
- Amino acids 41-57 of human Gli-3 protein.
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide from human GLI3.
Type of Immunogen: Synthetic peptide
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应用备注
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Approved: ELISA (1:6000 - 1:30000), ICC, IF, IHC, IHC-P (1.25 μg/mL), WB (1:500 - 1:2000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1.25 μg/mL. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- GLI3 (GLI Family Zinc Finger 3 (GLI3))
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别名
- GLI3
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背景
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Name/Gene ID: GLI3
Synonyms: GLI3, ACLS, GCPS, GLI-Kruppel family member GLI3, GLI3 full length protein, GLI3-190, GLI family zinc finger 3, Gli-3, GLI3FL, PAP-A, PAPA1, PHS, PPDIV, Transcriptional activator GLI3, PAPA, PAPB, GLI3 form of 190 kDa, Oncogene GLI3, Zinc finger protein GLI3 -
基因ID
- 2737
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UniProt
- P10071
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途径
- Hedgehog Signaling
抗原
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