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CD37 抗体 (FITC)

This FITC-conjugated 小鼠 单克隆 anti-CD37 antibody (Clone 4AIPO-24) specifically detects CD37 in FACS, ELISA, IF 和 IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN2144579
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CD37 抗体 (FITC) (ABIN2144579)

抗原

See all CD37 (TSPAN26) 抗体
CD37 (TSPAN26) (Tetraspanin 26 (TSPAN26))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD37 antibody is conjugated to FITC

应用范围

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Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

4AIPO-24
  • 产品特性

    4AIPO-24 reacts with CD37 (a.k.a. gp52-40 ), a 40-52 kDa molecule, which is strongly expressed on B cells from the pre-B cell stage, but not on plasma cells. It is also present at low levels on some T cells, monocytes and granulocytes. CD37 is a stable marker for malignancies derived from mature B cells, such as B-CLL, HCL and all types of B-NHL. CD37 is involved in signal transduction.CD37 is stable marker for malignancies derived from mature B cells: B-CLL. HCL and B-NHL.

    亚型

    IgG2b
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    实验流程

    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C
  • 抗原

    CD37 (TSPAN26) (Tetraspanin 26 (TSPAN26))

    别名

    CD37

    途径

    Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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