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CD37 抗体 (PE)

TSPAN26 适用: 人 FACS, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4AIPO-24 PE
产品编号 ABIN2144866
发货至: 中国
  • 抗原 See all CD37 (TSPAN26) 抗体
    CD37 (TSPAN26) (Tetraspanin 26 (TSPAN26))
    适用
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    宿主
    • 37
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    小鼠
    克隆类型
    • 38
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    单克隆
    标记
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    This CD37 antibody is conjugated to PE
    应用范围
    • 42
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    Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    产品特性
    4AIPO-24 reacts with CD37 (a.k.a. gp52-40 ), a 40-52 kDa molecule, which is strongly expressed on B cells from the pre-B cell stage, but not on plasma cells. It is also present at low levels on some T cells, monocytes and granulocytes. CD37 is a stable marker for malignancies derived from mature B cells, such as B-CLL, HCL and all types of B-NHL. CD37 is involved in signal transduction.CD37 is stable marker for malignancies derived from mature B cells: B-CLL. HCL and B-NHL.
    克隆位点
    4AIPO-24
    亚型
    IgG2b
    Top Product
    Discover our top product TSPAN26 Primary Antibody
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验流程
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
  • 抗原
    CD37 (TSPAN26) (Tetraspanin 26 (TSPAN26))
    别名
    CD37 (TSPAN26 产品)
    别名
    Tspan26 antibody, GP52-40 antibody, TSPAN26 antibody, CD37 antigen antibody, CD37 molecule antibody, Cd37 antibody, CD37 antibody
    途径
    Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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