PLK1 抗体 (pThr210)
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Quick Overview for PLK1 抗体 (pThr210) (ABIN214445)
抗原
See all PLK1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr210
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特异性
- Synthetic phospho peptide corresponding aa 205-214 of Human Polo-like kinase 1 (Plk1) protein.
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纯化方法
- Immunoaffinity purified
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免疫原
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Type of Immunogen: Native protein
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应用备注
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Approved: ELISA (1:3000 - 1:12000), IHC, IHC-P (10 μg/mL), WB (1:200 - 1:2000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.Western Blot: It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- PLK1 (Polo-Like Kinase 1 (PLK1))
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别名
- PLK1 / PLK-1
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背景
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Name/Gene ID: PLK1
Subfamily: Polo
Family: Protein Kinase
Synonyms: PLK1, Polo like kinase, Polo-like kinase (Drosophila), Polo-like kinase 1, STPK13, Polo (Drosophia)-like kinase, PLK, PLK-1 -
基因ID
- 5347
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UniProt
- P53350
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途径
- Cell Division Cycle, M Phase
抗原
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