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- 抗原 See all COL2 抗体
- COL2 (Collagen, Type II (COL2))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This COL2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 特异性
- This lot of antisera reacts to both human and bovine PTH C-Terminal region.
- 交叉反应
- 大鼠, 小鼠
- 交叉反应 (详细)
- This antibody that has been prepared bi immunoaffininty chromotography using immobilized antigens is followed by extensive cross-absorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins toremove any unwanted specifities.
- 纯化方法
- Purified by affinity chromatography.
- 过滤
- Sterile filtered
- 免疫原
- Collagen type II antibody was raised in rabbit using collagen type II from human knee cartilage and bovine nasal cartilage as the immunogen.
- Top Product
- Discover our top product COL2 Primary Antibody
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- 应用备注
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ELISA: 1:5,000-1:10,000, IHC: 1:50-1,200, IP: 1:100, WB: 1:5,000-1:10,000
Optimal conditions should be determined by the investigator. - 说明
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Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Sterile filtered liquid In 0.125 M Na2B4O7, pH 8.0, with 0.075 M NaCl, 0.005 M EDTA, and 0.01 % Sodium azide.
- 注意事项
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 注意事项
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. - 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
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TGF-β1, GDF-5, and BMP-2 stimulation induces chondrogenesis in expanded human articular chondrocytes and marrow-derived stromal cells." in: Stem cells (Dayton, Ohio), Vol. 33, Issue 3, pp. 762-73, (2015) (PubMed).
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TGF-β1, GDF-5, and BMP-2 stimulation induces chondrogenesis in expanded human articular chondrocytes and marrow-derived stromal cells." in: Stem cells (Dayton, Ohio), Vol. 33, Issue 3, pp. 762-73, (2015) (PubMed).
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- 抗原
- COL2 (Collagen, Type II (COL2))
- 别名
- Collagen Type II (COL2 产品)
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