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COL2 抗体

There is 1 publication for this product available. The 兔 多克隆 anti-COL2 antibody is suitable to detect COL2 in samples from 人. It has been validated for WB, IHC, ELISA 和 IP.
产品编号 ABIN2137989
发货至: 中国
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Quick Overview for COL2 抗体 (ABIN2137989)

抗原

See all COL2 抗体
COL2 (Collagen, Type II (COL2))

适用

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宿主

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克隆类型

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多克隆

标记

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This COL2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
  • 特异性

    This lot of antisera reacts to both human and bovine PTH C-Terminal region.

    交叉反应

    大鼠, 小鼠

    交叉反应 (详细)

    This antibody that has been prepared bi immunoaffininty chromotography using immobilized antigens is followed by extensive cross-absorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins toremove any unwanted specifities.

    纯化方法

    Purified by affinity chromatography.

    过滤

    Sterile filtered

    免疫原

    Collagen type II antibody was raised in rabbit using collagen type II from human knee cartilage and bovine nasal cartilage as the immunogen.
  • 应用备注

    ELISA: 1:5,000-1:10,000, IHC: 1:50-1,200, IP: 1:100, WB: 1:5,000-1:10,000
    Optimal conditions should be determined by the investigator.

    说明

    Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    Sterile filtered liquid In 0.125 M Na2B4O7, pH 8.0, with 0.075 M NaCl, 0.005 M EDTA, and 0.01 % Sodium azide.

    注意事项

    This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze/thaw cycles.
    Dilute only prior to immediate use.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
  • Murphy, Huey, Hu, Athanasiou: "TGF-β1, GDF-5, and BMP-2 stimulation induces chondrogenesis in expanded human articular chondrocytes and marrow-derived stromal cells." in: Stem cells (Dayton, Ohio), Vol. 33, Issue 3, pp. 762-73, (2015) (PubMed).

  • 抗原

    COL2 (Collagen, Type II (COL2))

    别名

    Collagen Type II
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