COL2 抗体
Quick Overview for COL2 抗体 (ABIN2137989)
抗原
See all COL2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This lot of antisera reacts to both human and bovine PTH C-Terminal region.
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交叉反应
- 大鼠, 小鼠
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交叉反应 (详细)
- This antibody that has been prepared bi immunoaffininty chromotography using immobilized antigens is followed by extensive cross-absorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins toremove any unwanted specifities.
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纯化方法
- Purified by affinity chromatography.
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过滤
- Sterile filtered
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免疫原
- Collagen type II antibody was raised in rabbit using collagen type II from human knee cartilage and bovine nasal cartilage as the immunogen.
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应用备注
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ELISA: 1:5,000-1:10,000, IHC: 1:50-1,200, IP: 1:100, WB: 1:5,000-1:10,000
Optimal conditions should be determined by the investigator. -
说明
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Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Sterile filtered liquid In 0.125 M Na2B4O7, pH 8.0, with 0.075 M NaCl, 0.005 M EDTA, and 0.01 % Sodium azide.
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注意事项
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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注意事项
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. -
储存条件
- 4 °C/-20 °C
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储存方法
- Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
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: "TGF-β1, GDF-5, and BMP-2 stimulation induces chondrogenesis in expanded human articular chondrocytes and marrow-derived stromal cells." in: Stem cells (Dayton, Ohio), Vol. 33, Issue 3, pp. 762-73, (2015) (PubMed).
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: "TGF-β1, GDF-5, and BMP-2 stimulation induces chondrogenesis in expanded human articular chondrocytes and marrow-derived stromal cells." in: Stem cells (Dayton, Ohio), Vol. 33, Issue 3, pp. 762-73, (2015) (PubMed).
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- COL2 (Collagen, Type II (COL2))
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别名
- Collagen Type II
抗原
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