Vimentin 抗体
Quick Overview for Vimentin 抗体 (ABIN213302)
抗原
See all Vimentin (VIM) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Recognizes a 57-60kD protein, human Vimentin. Shows no cross-reactivity with other closely related intermediate filament proteins including desmin and GFAP. Species cross-reactivity: monkey, bovine, canine, feline, chicken and rat. Does not recognize mouse.
-
纯化方法
- Purified
-
免疫原
-
Purified vimentin from porcine eye lens.
Type of Immunogen: Purified protein -
亚型
- IgG1
-
-
-
-
应用备注
-
Approved: ICC (5 - 10 μg/mL), IHC, IHC-P, WB (1:1000)
Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: 5-10 μg/mL, Can be used on formalin fixed paraffin embedded tissues with traditional high temperature, citrate buffer treatments. Prolonged formalin fixation can reduce antibody activity. Immunocytochemistry: 5-10 μg/mL. Positive control: Breast carcinoma tissue, colon, prostate, tonsil, tonsilar lymphoma, uterus and ureter. -
说明
-
Target Species of Antibody: Pig
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- PBS, pH 7.2, 0.1 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
-
-
- Vimentin (VIM)
-
别名
- Vimentin
-
背景
-
Name/Gene ID: VIM
Synonyms: VIM, Vimentin -
基因ID
- 7431
-
UniProt
- P08670
-
途径
- Caspase Cascade in Apoptosis
抗原
-