Paxillin 抗体
Quick Overview for Paxillin 抗体 (ABIN212824)
抗原
See all Paxillin (PXN) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes human Paxillin at 68kD and a band around 50kD, which is most likely a degradation product. Species cross-reactivity: Human, mouse, rat, chicken and bovine. Other species not tested.
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纯化方法
- Protein G purified
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免疫原
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GST fusion protein containing full-length human paxillin.
Type of Immunogen: Fusion protein -
亚型
- IgG1
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应用备注
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Approved: ELISA, ICC (5 - 10 μg/mL), IP, WB (0.5 - 2 μg/mL)
Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects paxillin in RIPA lysates from 3T3 fibroblasts using 20 μg per lane for minigels. Immunoprecipitation: 4 μg per 500 μg of cell lysate. Immunocytochemistry:5-10 μg/mL (gave positive immunostaining for paxillin in REF (rat embryo fibroblast) 52 cells fixed with 3.7 % paraformaldehyde for 5 minutes). -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- Paxillin (PXN)
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别名
- PXN / Paxillin
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背景
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Name/Gene ID: PXN
Synonyms: PXN, Paxillin -
基因ID
- 5829
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UniProt
- P49023
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途径
- MAPK Pathway, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
抗原
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