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PML 抗体 (AA 1-581)

The 小鼠 单克隆 anti-PML antibody (ABIN212004) specifically detects PML in WB 和 ICC. The antibody is reactive with 小鼠 samples.
产品编号 ABIN212004
发货至: 中国
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Quick Overview for PML 抗体 (AA 1-581) (ABIN212004)

抗原

See all PML 抗体
PML (Promyelocytic Leukemia (PML))

适用

  • 86
  • 33
  • 31
  • 2
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小鼠

宿主

  • 79
  • 10
小鼠

克隆类型

  • 69
  • 20
单克隆

标记

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This PML antibody is un-conjugated

应用范围

  • 69
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  • 2
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Western Blotting (WB), Immunocytochemistry (ICC)
  • 抗原表位

    • 13
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    AA 1-581

    特异性

    Recognizes mouse PML, Mr ~90-106kD, depending on degree of protein modification. Does not cross-react with human. MEF1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with This antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

    纯化方法

    Protein G purified

    免疫原

    6His fusion protein corresponding to aa1-581 of mouse PML (promyelocytic leukemia protein).

    Type of Immunogen: Fusion protein

    亚型

    IgG2b
  • 应用备注

    Approved: ICC, WB (0.5 - 1 μg/mL)

    Usage: Suitable for use in Western Blot and Immunocytochemistry. Western Blot: 0.5-1 μg/mL of this lot detected PML in RIPA lysates from mouse embryo fibroblast (MEF1) cells. Immunocytochemistry: An independent laboratory showed positive immunostaining for PML in MEF1 cells fixed with 4 % paraformaldehyde and permeabilized with 0.2 % Triton X-100.

    说明

    Target Species of Antibody: Mouse

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    PML (Promyelocytic Leukemia (PML))

    别名

    PML

    背景

    Name/Gene ID: PML
    Family: Transcription factor

    Synonyms: PML, PP8675, Promyelocytic leukemia, Protein PML, TRIM19, Promyelocytic leukemia protein, RING finger protein 71, MYL, RNF71

    基因ID

    5371

    UniProt

    P29590

    途径

    p53 Pathway, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus
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