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GAPDH 抗体

The 小鼠 单克隆 anti-GAPDH antibody (ABIN211932) specifically detects GAPDH in WB, ELISA, IF 和 ICC. The antibody is reactive with 人, 小鼠, 大鼠, 小鸡, 兔 和 Frog samples.
产品编号 ABIN211932
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for GAPDH 抗体 (ABIN211932)

抗原

See all GAPDH 抗体
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

适用

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人, 小鼠, 大鼠, 小鸡, 兔, Frog

宿主

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小鼠

克隆类型

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单克隆

标记

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This GAPDH antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
  • 特异性

    Recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit skeletal muscle. Also recognizes cardiac GAPDH. Species cross-reactivity: human, rat, mouse, chicken, frog and fish skeletal muscle. Does not react with GAPDH from E. coli. GAPDH enzyme is also detected in many non-muscle cell lines including HeLa, HCT-116, U937, and THP-1 cells.

    纯化方法

    Protein G purified

    免疫原

    Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit muscle.

    Type of Immunogen: Purified protein

    亚型

    IgG1
  • 应用备注

    Approved: ELISA (1:1000000), ICC (1:100 - 1:300), IF, WB (1:500)

    Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunofluorescence. ELISA: 1:1000000. Western Blot: 1:500. Detects on 10 μg of A431 lysates. Immunocytochemistry: 1:100-1:300. Dilute in PBS, 10 mg/mL BSA. Fixative: 4 % PFA, 0.02 % PBS-Triton X-100. Incubation time: 3 min at RT.

    说明

    Target Species of Antibody: Rabbit

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.1 % sodium azide. glycerol-free.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    avoid freezing

    储存条件

    4 °C

    储存方法

    4°C, avoid freezing.
  • 抗原

    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

    别名

    GAPDH

    背景

    Name/Gene ID: GAPDH

    Synonyms: GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit

    基因ID

    2597

    UniProt

    P04406
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