Histone H2B 抗体 (AA 118-126)
Quick Overview for Histone H2B 抗体 (AA 118-126) (ABIN211182)
抗原
See all Histone H2B 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 118-126
-
特异性
- Recognizes and is specific for Histone H2B, Mr ~15kD. An additional band at 23kD is detected in some samples, which likely corresponds to ubiquityl-Histone H2B. Species cross-reactivity: Human, chicken and Xenopus. Others not tested. Broad species cross-reactivity expected based on sequence homology.
-
预测反应
- Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (90%).
-
纯化方法
- Protein A purified
-
免疫原
-
KLH-conjugated, synthetic peptide corresponding to amino acids 118-126 (CGAVTKYTSSK) of human Histone H2B, with an N-terminal CG added for conjugation purposes. Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (90%).
Type of Immunogen: Synthetic peptide - KLH conjugated -
亚型
- IgG
-
-
-
-
应用备注
-
Approved: WB (1:1000 - 1:10000)
Usage: Suitable for use in Western Blot. Western Blot: 1:1000-1:10000 detected Histone H2B in acid extracts from either sodium butyrate or colcemid treated HeLa cells and 13-114 respectively), chicken core histone and recombinant Xenopus Histone H2B. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- 200 μLof 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
-
-
- Histone H2B
抗原
-