Histone H1 抗体 (hyperphosphorylated)
Quick Overview for Histone H1 抗体 (hyperphosphorylated) (ABIN211181)
抗原
See all Histone H1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- hyperphosphorylated
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特异性
- Recognizes phosphorylated histone H1 (32kD). Additional proteins (Mr 66 and 100kD) were detected. Species cross-reactivity: human, bovine.
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纯化方法
- Protein A purified
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免疫原
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Hyper-phosphorylated isoform of Tetrahymena Histone H1.
Type of Immunogen: Purified protein -
亚型
- IgG
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应用备注
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Approved: ICC (5 - 10 μg/mL), WB (1 - 2 μg/mL)
Usage: Suitable for use in Western Blot and Immunocytochemistry. Proteins were visualized using a goat anti-rabbit secondary antibody labeled with HRP. A chemiluminescence detection system was used. Immunocytochemistry: 5-10 μg/mL showed positive immunostaining for Histone H1 in mitotic HeLa cells fixed with 95 % ethanol, 5 % glacial acetic acid. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Histone H1
抗原
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